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Literature summary extracted from

  • Yu, Y.; Xu, W.; Wang, J.; Wang, L.; Yao, W.; Yang, Y.; Xu, Y.; Ma, F.; Du, Y.; Wang, Y.
    The Chinese wild grapevine (Vitis pseudoreticulata) E3 ubiquitin ligase Erysiphe necator-induced RING finger protein 1 (EIRP1) activates plant defense responses by inducing proteolysis of the VpWRKY11 transcription factor (2013), New Phytol., 200, 834-846.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.27 expressed in onion epidermal cells and Arabidopsis thaliana Vitis pseudoreticulata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.27 cell membrane
-
 Vitis pseudoreticulata
-
-
2.3.2.27 cytoplasm
-
 Vitis pseudoreticulata 5737
-
2.3.2.27 nucleus
-
 Vitis pseudoreticulata 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.27 26970
-
 x * 26970, calculated from amino acid sequence Vitis pseudoreticulata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.27 S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine Vitis pseudoreticulata
-
 [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.27 Vitis pseudoreticulata
-
 Chinese wild grapevine, cultivar Carignane
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.27 S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine
-
 Vitis pseudoreticulata [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.27 ? x * 26970, calculated from amino acid sequence Vitis pseudoreticulata

Synonyms

EC Number Synonyms Comment Organism
2.3.2.27 E3 ubiquitin ligase Erysiphe necator-induced RING finger protein 1
-
 Vitis pseudoreticulata
2.3.2.27 EIRP1
-
 Vitis pseudoreticulata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.2.27 Vitis pseudoreticulata calculated from amino acid sequence
-
 5.6

Expression

EC Number Organism Comment Expression
2.3.2.27 Vitis pseudoreticulata enzyme expression is induced by powdery mildew up

General Information

EC Number General Information Comment Organism
2.3.2.27 physiological function the enzyme activates plant defense responses by inducing proteolysis of the VpWRKY11 transcription factor. Enzyme overexpression in Arabidopsis confers enhanced resistance to the pathogens Golovinomyces cichoracearum and Pseudomonas syringae pv. tomato DC3000 Vitis pseudoreticulata