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Literature summary extracted from

  • Fresco-Taboada, A.; Serra, I.; Fernandez-Lucas, J.; Acebal, C.; Arroyo, M.; Terreni, M.; de la Mata, I.
    Nucleoside 2'-deoxyribosyltransferase from psychrophilic bacterium Bacillus psychrosaccharolyticus - preparation of an immobilized biocatalyst for the enzymatic synthesis of therapeutic nucleosides (2014), Molecules, 19, 11231-11249.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.6 expressed in Escherichia coli BL21(DE3) cells Peribacillus psychrosaccharolyticus

General Stability

EC Number General Stability Organism
2.4.2.6 the enzyme is stable when immobilized on PEI-agarose but shows no activity when immobilized on glyoxyl-agarose. The best stability is obtained using dextrane oxidized at 70% (w/v) Peribacillus psychrosaccharolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.6 NaCl activity decreases 25% in presence of 1.5 M NaCl Peribacillus psychrosaccharolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.6 Na+
-
 Peribacillus psychrosaccharolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.6 16000
-
 6 * 16000, SDS-PAGE Peribacillus psychrosaccharolyticus
2.4.2.6 91400
-
 gel filtration Peribacillus psychrosaccharolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Peribacillus psychrosaccharolyticus
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Peribacillus psychrosaccharolyticus CECT 4074
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Peribacillus psychrosaccharolyticus
-
-
-
2.4.2.6 Peribacillus psychrosaccharolyticus CECT 4074
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.6 Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration Peribacillus psychrosaccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2'-deoxyuridine + 5-trifluorothymine
-
 Peribacillus psychrosaccharolyticus 5-trifluorothymidine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + 5-trifluorothymine
-
 Peribacillus psychrosaccharolyticus CECT 4074 5-trifluorothymidine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + adenine
-
 Peribacillus psychrosaccharolyticus 2'-deoxyadenosine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + adenine
-
 Peribacillus psychrosaccharolyticus CECT 4074 2'-deoxyadenosine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + thymidine
-
 Peribacillus psychrosaccharolyticus 2'-deoxythymidine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + thymidine
-
 Peribacillus psychrosaccharolyticus CECT 4074 2'-deoxythymidine + uracil
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Peribacillus psychrosaccharolyticus 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Peribacillus psychrosaccharolyticus CECT 4074 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 additional information the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces Peribacillus psychrosaccharolyticus ?
-
 ?
2.4.2.6 additional information the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces Peribacillus psychrosaccharolyticus CECT 4074 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.6 homohexamer 6 * 16000, SDS-PAGE Peribacillus psychrosaccharolyticus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.6 NDT
-
 Peribacillus psychrosaccharolyticus
2.4.2.6 nucleoside 2'-deoxyribosyltransferase
-
 Peribacillus psychrosaccharolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.6 50
-
-
 Peribacillus psychrosaccharolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.6 4 90 the enzyme is stable up to 50°C during 15 min Peribacillus psychrosaccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.6 8
-
-
 Peribacillus psychrosaccharolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.6 6 10 the enzyme is stable in a pH range from 6.0 to 9.0, and retains more than 85% activity at pH 10.0 Peribacillus psychrosaccharolyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.6 Peribacillus psychrosaccharolyticus isoelectric focusing
-
 4.6