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Literature summary extracted from

  • Summers, R.; Mohanty, S.; Gopishetty, S.; Subramanian, M.
    Genetic characterization of caffeine degradation by bacteria and its potential applications (2015), Microb. Biotechnol., 8, 369-378.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.13.212 agriculture treatment of coffee waste with caffeine-degrading microorganisms (either wild type or recombinant) may transform the waste into a valuable by-product, rather than a waste stream, because a caffeine concentration in the waste greater than 1% makes it unsuitable as animal feed or as a biofuel feedstock Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.212 gene tmuM, genes involved in the caffeine C-8 oxidation pathway are located in a 25.2-kb genomic DNA fragment of CBB1, including cdhABC (coding for caffeine dehydrogenase) and tmuM (coding for TmuM) Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.212 43000
-
 x * 43000 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.212 1,3,7-trimethylurate + NADH + H+ + O2 Pseudomonas sp.
-
 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.212 Pseudomonas sp. I7ASS7 gene tmuM
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.212 1,3,7-trimethylurate + NADH + H+ + O2
-
 Pseudomonas sp. 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.212 ? x * 43000 Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.13.212 NADH-dependent trimethyluric acid monooxygenase
-
 Pseudomonas sp.
1.14.13.212 tmuM
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.212 NADH
-
 Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.14.13.212 metabolism two distinct caffeine metabolic pathways in bacteria: N-demethylation and C-8 oxidation, the NADH-dependent trimethyluric acid monooxygenase is involved in the latter catalyzing the second step of the C-8 oxidation pathway, overview Pseudomonas sp.