Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shah, G.R.; Wesener, S.R.; Cheng, Y.Q.
    Engineered production of tryprostatins in E. coli through reconstitution of a partial ftm biosynthetic gene cluster from Aspergillus sp. (2015), Jacobs J. Biotechnol. Bioeng., 2, 009.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.293 gene ftmD, encoded in the ftm gene cluster, recombinant expression in Escherichia coli strain BL21(DE3) Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.293 additional information engineered production of tryprostatins in Escherichia coli through reconstitution of a partial ftm biosynthetic gene cluster from Aspergillus sp., i.e. four genes, ftmABCD, on two compatible plasmids Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.293 S-adenosyl-L-methionine + 6-hydroxytryprostatin B Aspergillus fumigatus
-
 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.293 Aspergillus fumigatus Q4WAW6 Af293 R202L variant; isolates Af293, A1163 and BM939, gene ftmD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.293 S-adenosyl-L-methionine + 6-hydroxytryprostatin B
-
 Aspergillus fumigatus S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.293 ftmD
-
 Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.293 S-adenosyl-L-methionine
-
 Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.1.1.293 evolution all ftm genes are expressed almost equally well in both Af293 and BM939 strains. But a point mutation found in ftmD in the genome of Af293 causes an arginine to leucine substitution at position 202 of FtmD, resulting in a dramatic decrease of the catalytic efficiency of FtmD. This mutated form of FtmD appeared not functioning under physiological conditions in Af293 to produce any detectable levels of TPS-A or any downstream metabolites Aspergillus fumigatus