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Literature summary extracted from
- Purification and characterization of enzymes involved in desulfurization of dibezothiophene in fossil fuels (2001), J. Mol. Catal. B, 11, 1061-1064.
No PubMed abstract available
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.14.14.21 | purified enzyme DszC, hanging drop vapour diffusion method, using a reservoir suliton containing 30% polyethylene glycol, 0.1 M sodium citrate, pH 5.6, and 0.2 M ammonium acetate, 4°C, X-ray diffraction structure determination and analysis | Rhodococcus erythropolis |
| 1.14.14.22 | purified enzyme DszA, hanging drop vapour diffusion method, using a reservoir suliton containing 12% polyethylene glycol 400 and 0.2 M CaCl2, 4°C, X-ray diffraction structure determination and analysis | Rhodococcus erythropolis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.22 | 50000 | - |
2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
| 1.14.14.22 | 97000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |  |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus erythropolis D-1 | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis D-1 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.21 | Rhodococcus erythropolis | A0A0C6DRW4 | gene dszC | - |
| 1.14.14.21 | Rhodococcus erythropolis D-1 | A0A0C6DRW4 | gene dszC | - |
| 1.14.14.22 | Rhodococcus erythropolis | Q6WE15 | gene dszA | - |
| 1.14.14.22 | Rhodococcus erythropolis D-1 | Q6WE15 | gene dszA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.21 | native enzyme to homogeneity by hydrophobic interaction and anion exchange chromatography. and gel filtration | Rhodococcus erythropolis |
| 1.14.14.22 | native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | best substrate | Rhodococcus erythropolis | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | best substrate | Rhodococcus erythropolis D-1 | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Rhodococcus erythropolis | 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Rhodococcus erythropolis D-1 | 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis D-1 | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | high activity | Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis D-1 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | high activity | Rhodococcus erythropolis D-1 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | thioxanthen-9-one + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | best substrate | Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | best substrate | Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.22 | additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis D-1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.22 | homodimer | 2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | DBT monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.21 | dibenzothiophene monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.21 | dszC | - |
Rhodococcus erythropolis |
| 1.14.14.22 | DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.22 | dibenzothiophene sulfone monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.22 | dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 30 | - |
- |
Rhodococcus erythropolis |
| 1.14.14.22 | 35 | - |
- |
Rhodococcus erythropolis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 7 | - |
- |
Rhodococcus erythropolis |
| 1.14.14.22 | 7 | - |
- |
Rhodococcus erythropolis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.22 | FMNH2 | - |
Rhodococcus erythropolis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | metabolism | the enzyme is involved in the pathway of microbial dibenzothiophene desulfurization, overview | Rhodococcus erythropolis |
| 1.14.14.22 | metabolism | the enzyme is involved in the pathway of microbial dibenzothiophene desulfurization, overview | Rhodococcus erythropolis |
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