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Literature summary extracted from

  • Santos-Moriano, P.; Fernandez-Arrojo, L.; Poveda, A.; Jimenez-Barbero, J.; Ballesteros, A.; Plou, F.
    Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: Effect of reaction conditions (2015), J. Mol. Catal. B, 119, 18-25.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 45000
-
x * 45000, SDS-PAGE Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Zymomonas mobilis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.10 commercial preparation
-
Zymomonas mobilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 additional information three activities are displayed by the levansucrase from Zymomonas mobilis: formation of levan (polymerization), production of short-chain fructooligosaccharides (FOS), and sucrose hydrolysis. Several inulin-type FOS (1-kestose, nystose, 1F-fructosylnystose), neo-FOS (blastose, neokestose, neonystose) and levan-type FOS (6-kestose, 6,6-nystose) are synthesized by levansucrase, identification by mass spectroscopy and NMR spectrometry, e.g. of 6,6-nystose (i.e. Fru-beta(2->6)-Fru-beta(2->6)-Fru-beta(2->1)-alphaGlc). Assay method optimization, transfructosylation to hydrolysis ratio. , overview Zymomonas mobilis ?
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?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 ? x * 45000, SDS-PAGE Zymomonas mobilis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 LEV-Y
-
Zymomonas mobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 4 40 assay at, levan formation reaches the highest value at 4°C and 100 g/l sucrose. The increase of temperature to 40°C and fructose concentration of 600 g/l causes a significant decrease of the levan concentration and a higher production of short-chain fructooligosaccharides, an increase of the temperature also causes an enhancement of hydrolytic activity Zymomonas mobilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.10 30 65 activity range, profile overview Zymomonas mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 5.5
-
-
Zymomonas mobilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.10 4 6 activity range, no activity below pH 3.5 and above pH 6.5, profile, overview Zymomonas mobilis