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Literature summary extracted from

  • Angelaccio, S.; Di Salvo, M.; Parroni, A.; Di Bello, A.; Contestabile, R.; Pascarella, S.
    Structural stability of cold-adapted serine hydroxymethyltransferase, a tool for beta-hydroxy-alpha-amino acid biosynthesis (2014), J. Mol. Catal. B, 110, 171-177.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 formaldehyde inactivation Escherichia coli
2.1.2.1 formaldehyde inactivation Psychromonas ingrahamii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 additional information
-
 additional information steady-state kinetics Escherichia coli
2.1.2.1 additional information
-
 additional information steady-state kinetics Psychromonas ingrahamii
2.1.2.1 0.3
-
 L-serine pH 7.2, 20°C Escherichia coli
2.1.2.1 0.4
-
 L-serine pH 7.2, 20°C Psychromonas ingrahamii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Escherichia coli
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Psychromonas ingrahamii
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Psychromonas ingrahamii 37
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 additional information Psychromonas ingrahamii SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity ?
-
 ?
2.1.2.1 additional information Psychromonas ingrahamii 37 SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity ?
-
 ?
2.1.2.1 tetrahydrofolate + L-serine Escherichia coli
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine Psychromonas ingrahamii
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine Psychromonas ingrahamii 37
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Escherichia coli P0A825 gene glyA
-
2.1.2.1 Psychromonas ingrahamii A1SUU0 gene glyA
-
2.1.2.1 Psychromonas ingrahamii 37 A1SUU0 gene glyA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Escherichia coli tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Psychromonas ingrahamii tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Psychromonas ingrahamii 37 tetrahydrofolate + L-serine
-
 r
2.1.2.1 additional information SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity Psychromonas ingrahamii ?
-
 ?
2.1.2.1 additional information SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity Psychromonas ingrahamii 37 ?
-
 ?
2.1.2.1 tetrahydrofolate + L-serine
-
 Escherichia coli 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Psychromonas ingrahamii 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Psychromonas ingrahamii 37 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Escherichia coli
2.1.2.1 serine hydroxymethyltransferase
-
 Psychromonas ingrahamii
2.1.2.1 SHMT
-
 Escherichia coli
2.1.2.1 SHMT
-
 Psychromonas ingrahamii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 20
-
 assay at Escherichia coli
2.1.2.1 20
-
 assay at Psychromonas ingrahamii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 9.25
-
 L-serine pH 7.2, 20°C Psychromonas ingrahamii
2.1.2.1 13.17
-
 L-serine pH 7.2, 20°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 7.2
-
 assay at Escherichia coli
2.1.2.1 7.2
-
 assay at Psychromonas ingrahamii

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 5,10-methylenetetrahydrofolate
-
 Escherichia coli
2.1.2.1 5,10-methylenetetrahydrofolate
-
 Psychromonas ingrahamii
2.1.2.1 pyridoxal 5'-phosphate dependent on Escherichia coli
2.1.2.1 pyridoxal 5'-phosphate dependent on Psychromonas ingrahamii
2.1.2.1 tetrahydrofolate
-
 Escherichia coli
2.1.2.1 tetrahydrofolate
-
 Psychromonas ingrahamii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.2.1 23.13
-
 L-serine pH 7.2, 20°C Psychromonas ingrahamii
2.1.2.1 43.88
-
 L-serine pH 7.2, 20°C Escherichia coli