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Literature summary extracted from

  • Yang, L.; Liu, Q.; Liu, Z.; Yang, H.; Wang, J.; Li, X.; Yang, Y.
    Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling (2016), J. Integr. Plant Biol., 58, 67-80.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.27 expressed in Escherichia coli Rosetta cells Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.27 cytoplasm
-
 Arabidopsis thaliana 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.27 S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine Arabidopsis thaliana
-
 [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.27 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.27 glutathione Sepharose bead chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.27 S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine
-
 Arabidopsis thaliana [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.27 abscisic acid-insensitive RING protein 4
-
 Arabidopsis thaliana
2.3.2.27 AIRP4
-
 Arabidopsis thaliana
2.3.2.27 C3HC4-RING finger E3 ubiquitin ligase
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.3.2.27 Arabidopsis thaliana the transcription level can be induced by absisic acid (0.1 mM), heat (37°C), osmotic (300 mM mannitol) and oxidative stresses (20 mM H2O2) up

General Information

EC Number General Information Comment Organism
2.3.2.27 physiological function the enzyme acts as a positive regulator of absisic acid-mediated drought avoidance and a negative regulator of salt tolerance in Arabidopsis. Enzyme overexpression plants are hypersensitive to salt and osmotic stresses during seed germination, and show drought avoidance Arabidopsis thaliana