EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.91 | gene CsUGT78A14, DNA and amino acid sequence determination and analysis and sequence comparisons of the enzymes from different cultivars, phylogenetic analysis, expression profile of gene CsUGT78A14 is correlated with the accumulation patterns of F-glycosides, qRT-PCR, transcriptome sequencing of Camellia sinensis var. sinensis cvs. Nongkangzao, Huangjinya, and Quntizhong, overview | Camellia sinensis |
2.4.1.91 | gene CsUGT78A14, DNA and amino acid sequence determination and analysis, genome and transcriptome analysis and sequence comparisons of the enzymes from different cultivars, phylogenetic analysis, expression profile, qRT-PCR, transcriptome sequencing of Camellia sinensis var. sinensis cvs. Nongkangzao, Huangjinya, and Quntizhong, overview | Camellia sinensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.91 | Q373H | site-directed mutagenesis | Camellia sinensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.91 | additional information | - |
additional information | Michaelis-Menten kinetics | Camellia sinensis | |
2.4.1.91 | 0.0119 | - |
quercetin | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 0.0141 | - |
UDP-glucose | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 0.0238 | - |
myricetin | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 0.0309 | - |
kaempferol | pH 7.5, 30°C | Camellia sinensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.91 | UDP-glucose + a flavonol | Camellia sinensis | - |
UDP + a flavonol 3-O-beta-D-glucoside | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.91 | Camellia sinensis | A0A109P632 | var. assamica, gene CsUGT78A14 | - |
2.4.1.91 | Camellia sinensis | A0A109P632 | var. sinensis, cv. Nongkangzao, Huangjinya, and Quntizhong, gene CsUGT78A14 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.91 | additional information | enzyme expression profile, transcriptome analysis, the enzyme expression is correlated with the accumulation patterns of beta-glucogallin and the glycosylated flavonols, qRT-PCR | Camellia sinensis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.91 | additional information | product identification by mass spectrometry, quantification by HPLC. Sugar receptor and sugar donor specificity of enzyme CsUGT78A14, homology modelling analysis | Camellia sinensis | ? | - |
? | |
2.4.1.91 | UDP-glucose + a flavonol | - |
Camellia sinensis | UDP + a flavonol 3-O-beta-D-glucoside | - |
? | |
2.4.1.91 | UDP-glucose + apigenin | - |
Camellia sinensis | UDP + apigenin 3-O-beta-D-glucoside | - |
? | |
2.4.1.91 | UDP-glucose + cyanidin | - |
Camellia sinensis | UDP + cyanidin 3-O-beta-D-glucoside | - |
? | |
2.4.1.91 | UDP-glucose + epicatechin | - |
Camellia sinensis | UDP + epicatechin 3-O-beta-D-glucoside | - |
? | |
2.4.1.91 | UDP-glucose + eriodictyol | - |
Camellia sinensis | UDP + eriodictyol 3-O-beta-D-glucoside | - |
? | |
2.4.1.91 | UDP-glucose + kaempferol | - |
Camellia sinensis | UDP + kaempferol 3-O-beta-D-glucoside | - |
? | |
2.4.1.91 | UDP-glucose + myricetin | - |
Camellia sinensis | UDP + myricetin 3-O-beta-D-glucoside | - |
? | |
2.4.1.91 | UDP-glucose + naringenin | - |
Camellia sinensis | UDP + ? | - |
? | |
2.4.1.91 | UDP-glucose + quercetin | - |
Camellia sinensis | UDP + quercetin 3-O-beta-D-glucoside | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.91 | 3GlcT | - |
Camellia sinensis |
2.4.1.91 | CsUGT78A1 | - |
Camellia sinensis |
2.4.1.91 | flavonoid 3-O-glycosyltransferase | - |
Camellia sinensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.91 | 30 | - |
assay at | Camellia sinensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.91 | 3.11 | - |
myricetin | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 3.32 | - |
quercetin | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 3.61 | - |
UDP-glucose | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 6.75 | - |
kaempferol | pH 7.5, 30°C | Camellia sinensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.91 | 7.5 | - |
assay at | Camellia sinensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.91 | evolution | gene CsUGT78A14 and CsUGT78A15 share relatively higher identity with several flavonoid-3-O-glucosyltransferases, including VvGT1, VvGT6, AcF3GT1, and AcGaT, homology modelling analysis | Camellia sinensis |
2.4.1.91 | metabolism | the expression profiles of the genes CsUGT84A22, CsUGT78A14, and CsUGT78A15 are correlated with the accumulation patterns of beta-glucogallin and the glycosylated flavonols which indicates that the three CsUGT genes are involved in the biosynthesis of astringent compounds in Camellia sinensis | Camellia sinensis |
2.4.1.91 | additional information | the Gln373 residue plays a catalytically crucial role for flavonoid 3-O-glucosyltransferase activity. Enzyme expression profile, the enzyme expression is correlated with the accumulation patterns of beta-glucogallin and the glycosylated flavonols. Sequence comparison of UDP-glucosyltransferase CsUGT78A14 and UDP-galactosyltransferase CsUGT78A15 | Camellia sinensis |
2.4.1.91 | physiological function | the enzyme is responsible for the biosynthesis of flavonol 3-O-monoglycoside, the biosynthesis of astringent flavonol 3-O-monoglycoside compounds in tea plants | Camellia sinensis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.91 | 130.7 | - |
myricetin | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 218.4 | - |
kaempferol | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 256 | - |
UDP-glucose | pH 7.5, 30°C | Camellia sinensis | |
2.4.1.91 | 279 | - |
quercetin | pH 7.5, 30°C | Camellia sinensis |