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Literature summary extracted from

  • Park, S.J.; Kang, K.H.; Lee, H.; Park, A.R.; Yang, J.E.; Oh, Y.H.; Song, B.K.; Jegal, J.; Lee, S.H.; Lee, S.Y.
    Propionyl-CoA dependent biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates in metabolically engineered Escherichia coli (2013), J. Biotechnol., 165, 93-98.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.304 synthesis engineering of an in vivo polylactic acid biosynthesis system in Escherichia coli to synthesize 2-hydroxybutyrate-containing polyhydroxyalkanoate. Propionyl-CoA is used as precursor for 2-ketobutyrate that is converted into 2-hydroxybutyrate-CoA by the sequential actions of Lactococcus lactis (D)-2-hydroxybutyrate dehydrogenase (PanE) and PctCp and then 2-hydroxybutyrate-CoA is polymerized by an engineered PhaC1 from Pseudomonas sp. (mutant E130D/S325T/S477G/Q481K). The recombinant Escherichia coli expressing the system can be grown to 0.66 g/l and successfully produces P(70 mol% 3-hydroxybutyrate-co-18 mol% 3-hydroxybutyrate-co-12 mol% lactate) up to the polyhydroxyalkanoate content of 66 weight% from 20 g/l of glucose, 2 g/l of 3-hydroxybutyrate and 1 g/l of sodium propionate Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 expression in Escherichia coli Pseudomonas sp.
2.8.3.1 expressed in Escherichia coli XL1-Blue cells Anaerotignum propionicum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 E130D/S325T/S477G/Q481K engineered polyhydroxybutanoate synthase able to accept 2-hydroxyacyl-CoAs as substrates Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.1 acetyl-CoA + propanoate Anaerotignum propionicum
-
acetate + propanoyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Pseudomonas sp.
-
-
-
2.3.1.304 Pseudomonas sp. MBEL 6–19
-
-
-
2.8.3.1 Anaerotignum propionicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.1 acetyl-CoA + 2-hydroxybutanoate
-
Anaerotignum propionicum acetate + 2-hydroxybutyryl-CoA
-
?
2.8.3.1 acetyl-CoA + propanoate
-
Anaerotignum propionicum acetate + propanoyl-CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
2.8.3.1 PCT
-
Anaerotignum propionicum
2.8.3.1 propionyl-CoA transferase
-
Anaerotignum propionicum