EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.304 | synthesis | engineering of an in vivo polylactic acid biosynthesis system in Escherichia coli to synthesize 2-hydroxybutyrate-containing polyhydroxyalkanoate. Propionyl-CoA is used as precursor for 2-ketobutyrate that is converted into 2-hydroxybutyrate-CoA by the sequential actions of Lactococcus lactis (D)-2-hydroxybutyrate dehydrogenase (PanE) and PctCp and then 2-hydroxybutyrate-CoA is polymerized by an engineered PhaC1 from Pseudomonas sp. (mutant E130D/S325T/S477G/Q481K). The recombinant Escherichia coli expressing the system can be grown to 0.66 g/l and successfully produces P(70 mol% 3-hydroxybutyrate-co-18 mol% 3-hydroxybutyrate-co-12 mol% lactate) up to the polyhydroxyalkanoate content of 66 weight% from 20 g/l of glucose, 2 g/l of 3-hydroxybutyrate and 1 g/l of sodium propionate | Pseudomonas sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.304 | expression in Escherichia coli | Pseudomonas sp. |
2.8.3.1 | expressed in Escherichia coli XL1-Blue cells | Anaerotignum propionicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.304 | E130D/S325T/S477G/Q481K | engineered polyhydroxybutanoate synthase able to accept 2-hydroxyacyl-CoAs as substrates | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.1 | acetyl-CoA + propanoate | Anaerotignum propionicum | - |
acetate + propanoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.304 | Pseudomonas sp. | - |
- |
- |
2.3.1.304 | Pseudomonas sp. MBEL 619 | - |
- |
- |
2.8.3.1 | Anaerotignum propionicum | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.1 | acetyl-CoA + 2-hydroxybutanoate | - |
Anaerotignum propionicum | acetate + 2-hydroxybutyryl-CoA | - |
? | |
2.8.3.1 | acetyl-CoA + propanoate | - |
Anaerotignum propionicum | acetate + propanoyl-CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.3.1 | PCT | - |
Anaerotignum propionicum |
2.8.3.1 | propionyl-CoA transferase | - |
Anaerotignum propionicum |