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Literature summary extracted from

  • Kobylarz, M.J.; Grigg, J.C.; Sheldon, J.R.; Heinrichs, D.E.; Murphy, M.E.
    SbnG, a citrate synthase in Staphylococcus aureus: a new fold on an old enzyme (2014), J. Biol. Chem., 289, 33797-33807.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 expressed in Escherichia coli BL21(lambdaDE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.16 wild type enzyme bound to calcium by the hanging drop vapor diffusion method, using 0.2 M calcium acetate, 0.1 M imidazole (pH 9.0), and 4-6% (w/v) PEG 8000, and an active site variant E151Q in complex with oxaloacetate by the sitting drop vapor diffusion method, using 5% (v/v) tascimate (pH 7.0), 0.1 M HEPES (pH 7.0), 10% (w/v) PEG monomethyl ether 5000 and about 40 mM guanidine hydrochloride Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.16 D177A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
2.3.3.16 E151Q the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
2.3.3.16 E46Q the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
2.3.3.16 H47A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
2.3.3.16 H96A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus
2.3.3.16 R72A the mutant shows reduced activity compared to the wild type enzyme Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 Ca2+
-
 Staphylococcus aureus
2.3.3.16 Mg2+
-
 Staphylococcus aureus
2.3.3.16 additional information not inhibited by EDTA Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 28000
-
 6 * 28000, SDS-PAGE Staphylococcus aureus
2.3.3.16 171000
-
 multi-angle light scattering Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate Staphylococcus aureus
-
 citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Staphylococcus aureus A0A0H3K9Z0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 HisTrap column chromatography and Source 15Q column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate
-
 Staphylococcus aureus citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 homohexamer 6 * 28000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 SbnG
-
 Staphylococcus aureus