Literature summary extracted from

Giganti, D.; Alegre-Cebollada, J.; Urresti, S.; Albesa-Jove, D.; Rodrigo-Unzueta, A.; Comino, N.; Kachala, M.; Lopez-Fernandez, S.; Svergun, D.I.; Fernandez, J.M.; Guerin, M.E.
Conformational plasticity of the essential membrane-associated mannosyltransferase PimA from mycobacteria (2013), J. Biol. Chem., 288, 29797-29808.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.1.345	expressed in Escherichia coli BL3 cells	Mycolicibacterium smegmatis
2.4.1.345	expression in Escherichia coli	Mycolicibacterium smegmatis

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.4.1.345	additional information	in a polyprotein containing PimA flanked by four copies of the I27 protein, which provides a mechanical fingerprint, PimA exhibits weak mechanical stability albeit displaying beta-sheet topology expected to unfold at much higher forces. PimA unfolds following heterogeneous multiple step mechanical unfolding pathways at low force akin to molten globule states. The open and closed conformations of the GT-B glycosyltransferase are largely present in solution, and in addition, PimA experiences remarkable flexibility that corresponds to the N-terminal Rossmann fold domain	Mycolicibacterium smegmatis

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.4.1.345	membrane	-	Mycolicibacterium smegmatis	16020	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.1.345	GDP-mannose + 1-phosphatidyl-myo-inositol	Mycolicibacterium smegmatis	-	?	-	?
2.4.1.345	GDP-mannose + 1-phosphatidyl-myo-inositol	Mycolicibacterium smegmatis ATCC 700084	-	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.345	Mycolicibacterium smegmatis	A0QWG6	-	-
2.4.1.345	Mycolicibacterium smegmatis ATCC 700084	A0QWG6	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.1.345	Talon resin column chromatpgraphy and Superdex 200 gel filtration	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.345	GDP-mannose + 1-phosphatidyl-myo-inositol	-	Mycolicibacterium smegmatis	?	-	?
2.4.1.345	GDP-mannose + 1-phosphatidyl-myo-inositol	-	Mycolicibacterium smegmatis ATCC 700084	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.1.345	MSMEG_2935	-	Mycolicibacterium smegmatis
2.4.1.345	phosphatidyl-myo-inositol mannosyltransferase A	-	Mycolicibacterium smegmatis
2.4.1.345	PimA	-	Mycolicibacterium smegmatis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)