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Literature summary extracted from

  • Angata, T.; Nakata, D.; Matsuda, T.; Kitajima, K.; Troy, F.A.
    Biosynthesis of KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid). Identification and characterization of a KDN-9-phosphate synthetase activity from trout testis (1999), J. Biol. Chem., 274, 22949-22956.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.132 EDTA the enzyme is active in the absence of exogenously added divalent cations but lost all activity when incubated before assay with 5 mM EDTA. The lost enzyme activity is restored to 2.4–3 times higher levels of the original one by the addition of 1 mM MnCl2, CoCl2, or NiCl2 and to one-third the original activity by 1 mM MgCl2. The other cations (Ca2+, Fe2+, Cu2+, and Zn2+) do not restore enzyme activity Oncorhynchus mykiss
2.5.1.132 NaCl activity is reduced by about 50% in the presence of 150 mM NaCl compared with the activity without added NaCl Oncorhynchus mykiss

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.132 3.66
-
 D-mannose 6-phosphate pH 7.0, 25°C Oncorhynchus mykiss

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.132 Mn2+ maximal activity in presence of Mn2+ Oncorhynchus mykiss

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.132 80000
-
 gel filtration Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O Oncorhynchus mykiss the enzyme participates in the biosynthesis of the sialic acid 3-deoxy-D-glycero-D-galacto-nononate 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.132 Oncorhynchus mykiss
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.132
-
 Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.132 liver specific activity is 33fold lower than in testis Oncorhynchus mykiss
-
2.5.1.132 testis
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.132 0.0009
-
 pH 7.0, 25°C, the 50fold purified enzyme from testis is unstable, preventing further purification of this enzyme activity Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O the enzyme participates in the biosynthesis of the sialic acid 3-deoxy-D-glycero-D-galacto-nononate Oncorhynchus mykiss 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 ?
2.5.1.132 phosphoenolpyruvate + D-mannose 6-phosphate + H2O phosphoenolpyruvate i.e. 2-(phosphonooxy)-2-propenoate Oncorhynchus mykiss 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.132 2-keto-3-deoxy-D-glycero-D-galacto-9-phosphonononic acid synthase
-
 Oncorhynchus mykiss
2.5.1.132 KDN-9-phosphate synthetase
-
 Oncorhynchus mykiss

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.132 7 8
-
 Oncorhynchus mykiss