Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Haigler, B.E.; Nishino, S.F.; Spain, J.C.
    Biodegradation of 4-methyl-5-nitrocatechol by Pseudomonas sp. strain DNT (1994), J. Bacteriol., 176, 3433-3437.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.210 4-methyl-5-nitrocatechol + O2 + NADPH + H+ Pseudomonas sp.
-
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
?
1.14.13.210 4-methyl-5-nitrocatechol + O2 + NADPH + H+ Pseudomonas sp. DNT
-
2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.210 Pseudomonas sp.
-
-
-
1.14.13.210 Pseudomonas sp. DNT
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.210 4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O mechanism of removal of the nitro group from 4-methyl-5-nitrocatechol and subsequent reactions leading to ring fission, overview Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.210 0.03
-
strain DNT grown on yeast extract, pH and temperature not specified in the publication Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.210 4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
Pseudomonas sp. 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
?
1.14.13.210 4-methyl-5-nitrocatechol + O2 + NADPH + H+
-
Pseudomonas sp. DNT 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.210 MNC oxygenase
-
Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.210 NADPH
-
Pseudomonas sp.