EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.21 | expressed in Escherichia coli | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.21 | 0.09 | - |
ADP-alpha-D-glucose | isoform GBSSI, with maltobiose as cosubstrate, at pH 8.5 and 30°C | Hordeum vulgare | |
2.4.1.21 | 0.31 | - |
ADP-alpha-D-glucose | isoform SSIV, with maltotriose as cosubstrate, at pH 8.5 and 30°C | Hordeum vulgare | |
2.4.1.21 | 0.44 | - |
ADP-alpha-D-glucose | isoform SSIIIb, with maltotriose as cosubstrate, at pH 8.5 and 30°C | Hordeum vulgare | |
2.4.1.21 | 0.63 | - |
ADP-alpha-D-glucose | isoform SSI with maltotriose as cosubstrate, at pH 8.5 and 30°C | Hordeum vulgare | |
2.4.1.21 | 46.32 | - |
maltopentaose | isoform GBSSI, at pH 8.5 and 30°C | Hordeum vulgare |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.21 | amyloplast starch grain | - |
Hordeum vulgare | 9568 | - |
2.4.1.21 | chloroplast | - |
Hordeum vulgare | 9507 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.21 | 59000 | - |
x * 59000, isoform GBSSI, calculated from amino acid sequence | Hordeum vulgare |
2.4.1.21 | 67500 | - |
x * 67500, isoform SSI, calculated from amino acid sequence | Hordeum vulgare |
2.4.1.21 | 81600 | - |
x * 81600, isoform SSIIa, calculated from amino acid sequence | Hordeum vulgare |
2.4.1.21 | 98300 | - |
x * 98300, isoform SSIV, calculated from amino acid sequence | Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.21 | ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n | Hordeum vulgare | - |
ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.21 | Hordeum vulgare | F2CUZ5 | - |
- |
2.4.1.21 | Hordeum vulgare | G1FUP7 | - |
- |
2.4.1.21 | Hordeum vulgare | I1W6L2 | - |
- |
2.4.1.21 | Hordeum vulgare | P09842 | - |
- |
2.4.1.21 | Hordeum vulgare | Q8H1Y8 | - |
- |
2.4.1.21 | Hordeum vulgare | Q9M5A3 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.21 | endosperm | - |
Hordeum vulgare | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.21 | ADP-alpha-D-glucose + 3-phosphomaltose | - |
Hordeum vulgare | ADP + ? | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + amylopectin | - |
Hordeum vulgare | ADP + ? | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + amylopectin | high activity | Hordeum vulgare | ADP + ? | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + glycogen | - |
Hordeum vulgare | ADP + ? | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + glycogen | high activity | Hordeum vulgare | ADP + ? | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + maltoheptaose | - |
Hordeum vulgare | ADP + maltooctaose | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + maltohexaose | - |
Hordeum vulgare | ADP + maltoheptaose | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + maltooctaose | - |
Hordeum vulgare | ADP + maltononaose | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + maltopentaose | - |
Hordeum vulgare | ADP + maltohexaose | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + maltose | - |
Hordeum vulgare | ADP + maltotriose | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + maltotetraose | - |
Hordeum vulgare | ADP + maltopentaose | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + maltotriose | - |
Hordeum vulgare | ADP + maltotetraose | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + soluble starch | - |
Hordeum vulgare | ADP + ? | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + soluble starch | high activity | Hordeum vulgare | ADP + ? | - |
? | |
2.4.1.21 | ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n | - |
Hordeum vulgare | ADP + [(1->4)-alpha-D-glucosyl]n+1 | - |
? | |
2.4.1.21 | additional information | no significant activity with D-glucose as acceptor substrate | Hordeum vulgare | ? | - |
? | |
2.4.1.21 | additional information | UDP-alpha-D-glucose is a substrate for isoforms GBSSI, SSI, and SSII but not for SSIII and SSIV | Hordeum vulgare | ? | - |
? | |
2.4.1.21 | additional information | UDP-alpha-D-glucose is a substrate for isoforms GBSSI, SSI, and SSIIa but not for SSIII and SSIV | Hordeum vulgare | ? | - |
? | |
2.4.1.21 | additional information | UDP-alpha-D-glucose is not a substrate for isoform SSIV | Hordeum vulgare | ? | - |
? | |
2.4.1.21 | additional information | UDP-alpha-D-glucose is not a substrate for SSIII | Hordeum vulgare | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.21 | ? | x * 59000, isoform GBSSI, calculated from amino acid sequence | Hordeum vulgare |
2.4.1.21 | ? | x * 67500, isoform SSI, calculated from amino acid sequence | Hordeum vulgare |
2.4.1.21 | ? | x * 81600, isoform SSIIa, calculated from amino acid sequence | Hordeum vulgare |
2.4.1.21 | ? | x * 98300, isoform SSIV, calculated from amino acid sequence | Hordeum vulgare |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.21 | GBSSI | isoform | Hordeum vulgare |
2.4.1.21 | SSI | isoform | Hordeum vulgare |
2.4.1.21 | SSIIa | isoform | Hordeum vulgare |
2.4.1.21 | SSIIIa | isoform | Hordeum vulgare |
2.4.1.21 | SSIIIb | isoform | Hordeum vulgare |
2.4.1.21 | SSIV | isoform | Hordeum vulgare |
2.4.1.21 | starch synthase | - |
Hordeum vulgare |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.21 | 39.8 | - |
temperature at which 50% of the activity of isoform GBSSI remains after 15 min incubation | Hordeum vulgare |
2.4.1.21 | 40.1 | - |
temperature at which 50% of the activity of isoform SSIIa remains after 15 min incubation | Hordeum vulgare |
2.4.1.21 | 43.8 | - |
temperature at which 50% of the activity of isoform SSI remains after 15 min incubation | Hordeum vulgare |
2.4.1.21 | 46.3 | - |
temperature at which 50% of the activity of isoform SSIIIb remains after 15 min incubation | Hordeum vulgare |
2.4.1.21 | 47.4 | - |
temperature at which 50% of the activity of isoform SSIV remains after 15 min incubation | Hordeum vulgare |