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Literature summary extracted from

  • Chong, B.F.; Harrison, M.D.; OHara, I.M.
    Stability of endoglucanases from mesophilic fungus and thermophilic bacterium in acidified polyols (2014), Enzyme Microb. Technol., 61-62, 55-60.
    View publication on PubMed

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 Ethylene glycol 95.6% glycerol, plus 0.1% H2SO4, 36% residual activity Thermotoga maritima
3.2.1.4 Ethylene glycol 95.6%, plus 0.1% H2SO4, 36% residual activity Trichoderma longibrachiatum
3.2.1.4 Glycerol 96%, plus 0.1% H2SO4, 35% residual activity Thermotoga maritima
3.2.1.4 Glycerol 96%, plus 0.1% H2SO4, 80% residual activity Trichoderma longibrachiatum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermotoga maritima
-
-
-
3.2.1.4 Trichoderma longibrachiatum Q12714
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 commercial preparation
-
 Thermotoga maritima
-
3.2.1.4 commercial preparation
-
 Trichoderma longibrachiatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 127.2
-
 presence of 96% glycerol, pH 4.8, 4°C Thermotoga maritima
3.2.1.4 139.6
-
 pH 4.8, 4°C Thermotoga maritima
3.2.1.4 264.7
-
 presence of 96% glycerol, pH 4.8, 4°C Trichoderma longibrachiatum
3.2.1.4 331.9
-
 pH 4.8, 4°C Trichoderma longibrachiatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Thermotoga maritima ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Trichoderma longibrachiatum ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 80
-
 presence of 96%, plus 0.1% H2SO4, half-life 4.5 min Thermotoga maritima
3.2.1.4 80
-
 presence of 96%glycerol, plus 0.1% H2SO4, half-life 4.5 min Trichoderma longibrachiatum
3.2.1.4 90
-
 5 min, 87% residiual activity Thermotoga maritima
3.2.1.4 90
-
 5 min, 87% residiual activity Trichoderma longibrachiatum
3.2.1.4 100
-
 5 min, 5% residual activity Thermotoga maritima
3.2.1.4 100
-
 5 min, 75% residual activity Trichoderma longibrachiatum