Literature summary extracted from
Cui, Z.; Wang, Y.; Lei, N.; Wang, K.; Zhu, T.
Botrytis cinerea chitin synthase BcChsVI is required for normal growth and pathogenicity (2013), Curr. Genet., 59, 119-128.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.1.16 |
gene BcChs6, cloning in Escherichia coli strain DH5alpha, Agrobacterium-mediated transformation of Bortrytis cinerea spores for gene disruption |
Botrytis cinerea |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.4.1.16 |
additional information |
the gene is disrupted through Agrobacterium tumefaciens-mediated transformation |
Botrytis cinerea |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.16 |
Botrytis cinerea |
Q5BNB8 |
a haploid strain, gene BcChs6 or BC1G_15936.1 |
- |
2.4.1.16 |
Botrytis cinerea B05.10 |
Q5BNB8 |
a haploid strain, gene BcChs6 or BC1G_15936.1 |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.4.1.16 |
mycelium |
- |
Botrytis cinerea |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.4.1.16 |
BcChsVI |
- |
Botrytis cinerea |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.4.1.16 |
malfunction |
the Bcchs6 disruption mutant exhibits a 45.5% increasing in its chitin content when compared with the wild-type strain. In Bcchs6 mutant the expression of BcChs6 is significantly decreased, while the expression of genes BcChs2 and BcChs3a is increased when compared with wild-type. It is probable that the disruption of gene Bcchs6 provokes a compensatory mechanism regulatingthe cellular response to cell wall damage. The radial growth of Bcchs6 mutant is drastically reduced when 50% solute is removed from the regular PDA medium, and they are more sensitive to Calcofluor white and other cell wall disturbing chemicals. Pathogenicity assays on tomato leaves indicate that the mutant is significantly reduced in their ability to cause disease. The radial lesion produced by Bcchs6 mutant is almost not detected even at 6 days after inoculation, indicating the pathogenicity is drastically reduced in Bcchs6 mutant |
Botrytis cinerea |
2.4.1.16 |
physiological function |
enzyme BcChsVI is necessary for proper hyphal growth and pathogenicity of Botrytis cinerea on tomato leaves, the radial lesion induced by the wild-type strain B05.10 spread rapidly and totally invaded the leaf at 4 days post inoculation |
Botrytis cinerea |