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Literature summary extracted from

  • Markert, B.; Stolzenberger, J.; Brautaset, T.; Wendisch, V.F.
    Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus (2014), BMC Microbiol., 14, 7.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1 expressed in Escherichia coli BL21(DE3) cells Bacillus methanolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 ADP
-
Bacillus methanolicus
2.2.1.1 ATP
-
Bacillus methanolicus
2.2.1.1 EDTA
-
Bacillus methanolicus
2.2.1.1 Ni2+
-
Bacillus methanolicus
2.2.1.1 phosphate buffer 20 mM phosphate buffer shows an inhibitory effect of the enzyme activity of approximately 40% Bacillus methanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.12
-
D-ribose 5-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 0.15
-
D-xylulose 5-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 0.23
-
D-xylulose 5-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 0.25
-
D-fructose 6-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 0.25
-
D-ribose 5-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 0.67
-
D-glyceraldehyde 3-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 0.72
-
D-fructose 6-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 0.92
-
D-glyceraldehyde 3-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Ca2+ activates Bacillus methanolicus
2.2.1.1 Co2+ activates Bacillus methanolicus
2.2.1.1 Cu2+ activates Bacillus methanolicus
2.2.1.1 Mg2+ activates Bacillus methanolicus
2.2.1.1 Mn2+ activates Bacillus methanolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 73000
-
4 * 73000, SDS-PAGE Bacillus methanolicus
2.2.1.1 280000
-
gel filtration Bacillus methanolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate Bacillus methanolicus
-
D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
?
2.2.1.1 D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate Bacillus methanolicus MGA3
-
D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
?
2.2.1.1 D-ribose 5-phosphate + D-xylulose 5-phosphate Bacillus methanolicus
-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
?
2.2.1.1 D-ribose 5-phosphate + D-xylulose 5-phosphate Bacillus methanolicus MGA3
-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Bacillus methanolicus
-
-
-
2.2.1.1 Bacillus methanolicus MGA3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 Ni-NTA column chromatography Bacillus methanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate
-
Bacillus methanolicus D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
?
2.2.1.1 D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate
-
Bacillus methanolicus MGA3 D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
?
2.2.1.1 D-ribose 5-phosphate + D-xylulose 5-phosphate
-
Bacillus methanolicus sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
?
2.2.1.1 D-ribose 5-phosphate + D-xylulose 5-phosphate
-
Bacillus methanolicus MGA3 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.2.1.1 homotetramer 4 * 73000, SDS-PAGE Bacillus methanolicus

Synonyms

EC Number Synonyms Comment Organism
2.2.1.1 TKT
-
Bacillus methanolicus
2.2.1.1 TKTc isoform Bacillus methanolicus
2.2.1.1 TKTp isoform Bacillus methanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.1 62
-
-
Bacillus methanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.1 50
-
the enzyme isoforms TKTc and TKTp remain stable up to 50°C for at least 2 h. Upon pre-incubation at 60°C the catalytic activity is reduced for both enzymes to approximately 60% within 10 min and then remains stable at this level. Incubation at 70°C leads to a complete loss of activity for TKTc after 4 min, for TKTp after 30 min of incubation Bacillus methanolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.1 13
-
D-ribose 5-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 21
-
D-ribose 5-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 40
-
D-xylulose 5-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 48
-
D-glyceraldehyde 3-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 54
-
D-xylulose 5-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 82
-
D-fructose 6-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 99
-
D-glyceraldehyde 3-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 112
-
D-fructose 6-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 7.2 7.4
-
Bacillus methanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
Bacillus methanolicus

General Information

EC Number General Information Comment Organism
2.2.1.1 metabolism transketolase is a key enzyme of the pentose phosphate pathway, the Calvin cycle and the ribulose monophosphate cycle Bacillus methanolicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.1 71
-
D-glyceraldehyde 3-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 84
-
D-ribose 5-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 108
-
D-glyceraldehyde 3-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 109
-
D-ribose 5-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 115
-
D-fructose 6-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 231
-
D-xylulose 5-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus
2.2.1.1 264
-
D-xylulose 5-phosphate isoform TKTc, at pH 7.5 and 55°C Bacillus methanolicus
2.2.1.1 448
-
D-fructose 6-phosphate isoform TKTp, at pH 7.7 and 55°C Bacillus methanolicus