EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.1 | expressed in Escherichia coli BL21(DE3) cells | Bacillus methanolicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.1 | ADP | - |
Bacillus methanolicus | |
2.2.1.1 | ATP | - |
Bacillus methanolicus | |
2.2.1.1 | EDTA | - |
Bacillus methanolicus | |
2.2.1.1 | Ni2+ | - |
Bacillus methanolicus | |
2.2.1.1 | phosphate buffer | 20 mM phosphate buffer shows an inhibitory effect of the enzyme activity of approximately 40% | Bacillus methanolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.1 | 0.12 | - |
D-ribose 5-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 0.15 | - |
D-xylulose 5-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 0.23 | - |
D-xylulose 5-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 0.25 | - |
D-fructose 6-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 0.25 | - |
D-ribose 5-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 0.67 | - |
D-glyceraldehyde 3-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 0.72 | - |
D-fructose 6-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 0.92 | - |
D-glyceraldehyde 3-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.1 | Ca2+ | activates | Bacillus methanolicus | |
2.2.1.1 | Co2+ | activates | Bacillus methanolicus | |
2.2.1.1 | Cu2+ | activates | Bacillus methanolicus | |
2.2.1.1 | Mg2+ | activates | Bacillus methanolicus | |
2.2.1.1 | Mn2+ | activates | Bacillus methanolicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.2.1.1 | 73000 | - |
4 * 73000, SDS-PAGE | Bacillus methanolicus |
2.2.1.1 | 280000 | - |
gel filtration | Bacillus methanolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.1 | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | Bacillus methanolicus | - |
D-erythrose 4-phosphate + D-xylulose 5-phosphate | - |
? | |
2.2.1.1 | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | Bacillus methanolicus MGA3 | - |
D-erythrose 4-phosphate + D-xylulose 5-phosphate | - |
? | |
2.2.1.1 | D-ribose 5-phosphate + D-xylulose 5-phosphate | Bacillus methanolicus | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
2.2.1.1 | D-ribose 5-phosphate + D-xylulose 5-phosphate | Bacillus methanolicus MGA3 | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.1 | Bacillus methanolicus | - |
- |
- |
2.2.1.1 | Bacillus methanolicus MGA3 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.2.1.1 | Ni-NTA column chromatography | Bacillus methanolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.1 | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
Bacillus methanolicus | D-erythrose 4-phosphate + D-xylulose 5-phosphate | - |
? | |
2.2.1.1 | D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
Bacillus methanolicus MGA3 | D-erythrose 4-phosphate + D-xylulose 5-phosphate | - |
? | |
2.2.1.1 | D-ribose 5-phosphate + D-xylulose 5-phosphate | - |
Bacillus methanolicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
2.2.1.1 | D-ribose 5-phosphate + D-xylulose 5-phosphate | - |
Bacillus methanolicus MGA3 | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.2.1.1 | homotetramer | 4 * 73000, SDS-PAGE | Bacillus methanolicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.1 | TKT | - |
Bacillus methanolicus |
2.2.1.1 | TKTc | isoform | Bacillus methanolicus |
2.2.1.1 | TKTp | isoform | Bacillus methanolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.1 | 62 | - |
- |
Bacillus methanolicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.1 | 50 | - |
the enzyme isoforms TKTc and TKTp remain stable up to 50°C for at least 2 h. Upon pre-incubation at 60°C the catalytic activity is reduced for both enzymes to approximately 60% within 10 min and then remains stable at this level. Incubation at 70°C leads to a complete loss of activity for TKTc after 4 min, for TKTp after 30 min of incubation | Bacillus methanolicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.1 | 13 | - |
D-ribose 5-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 21 | - |
D-ribose 5-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 40 | - |
D-xylulose 5-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 48 | - |
D-glyceraldehyde 3-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 54 | - |
D-xylulose 5-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 82 | - |
D-fructose 6-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 99 | - |
D-glyceraldehyde 3-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 112 | - |
D-fructose 6-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.2.1.1 | 7.2 | 7.4 | - |
Bacillus methanolicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.1 | thiamine diphosphate | - |
Bacillus methanolicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.2.1.1 | metabolism | transketolase is a key enzyme of the pentose phosphate pathway, the Calvin cycle and the ribulose monophosphate cycle | Bacillus methanolicus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.2.1.1 | 71 | - |
D-glyceraldehyde 3-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 84 | - |
D-ribose 5-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 108 | - |
D-glyceraldehyde 3-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 109 | - |
D-ribose 5-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 115 | - |
D-fructose 6-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 231 | - |
D-xylulose 5-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 264 | - |
D-xylulose 5-phosphate | isoform TKTc, at pH 7.5 and 55°C | Bacillus methanolicus | |
2.2.1.1 | 448 | - |
D-fructose 6-phosphate | isoform TKTp, at pH 7.7 and 55°C | Bacillus methanolicus |