EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.21 | gene dszC, recombinant expression in Escherichia coli strain BL21(DE3) | Rhodococcus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.21 | 45000 | - |
6 * 45000, about, recombinant enzyme, SDS-PAGE | Rhodococcus sp. |
1.14.14.21 | 294000 | - |
recombinant enzyme, gel filtration | Rhodococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus sp. | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | Rhodococcus sp. IIPS7 | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.21 | Rhodococcus sp. | Q3HSE4 | gene dszC | - |
1.14.14.21 | Rhodococcus sp. IIPS7 | Q3HSE4 | gene dszC | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.21 | recombinant enzyme 39fold from Escherichia coli strain BL21(DE3) by affinity chromatography | Rhodococcus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.21 | 3 | 8 | purified recombinant enzyme, pH 7.0, 30°C | Rhodococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus sp. | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus sp. IIPS7 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.21 | homohexamer | 6 * 45000, about, recombinant enzyme, SDS-PAGE | Rhodococcus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.21 | DBT monooxygenase | - |
Rhodococcus sp. |
1.14.14.21 | dibenzothiophene monooxygenase | - |
Rhodococcus sp. |
1.14.14.21 | dszC | - |
Rhodococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.21 | 30 | - |
- |
Rhodococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.21 | 7 | - |
- |
Rhodococcus sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.21 | metabolism | dibenzothiophene monooxygenase is the first enzyme involved in the degradation of dibenzothiophene | Rhodococcus sp. |