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Literature summary extracted from

  • Gupta, N.; Adhikari, D.K.; Stobdan, T.; Roychoudhury, P.K.; Deb, J.K.
    Purification of dibenzothiophene monooxygenase from a recombinant Escherichia coli (2007), Biotechnol. Lett., 29, 1465-1468.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.21 gene dszC, recombinant expression in Escherichia coli strain BL21(DE3) Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.21 45000
-
6 * 45000, about, recombinant enzyme, SDS-PAGE Rhodococcus sp.
1.14.14.21 294000
-
recombinant enzyme, gel filtration Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Rhodococcus sp.
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Rhodococcus sp. IIPS7
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.21 Rhodococcus sp. Q3HSE4 gene dszC
-
1.14.14.21 Rhodococcus sp. IIPS7 Q3HSE4 gene dszC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.21 recombinant enzyme 39fold from Escherichia coli strain BL21(DE3) by affinity chromatography Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.21 3 8 purified recombinant enzyme, pH 7.0, 30°C Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
Rhodococcus sp. dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
Rhodococcus sp. IIPS7 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.21 homohexamer 6 * 45000, about, recombinant enzyme, SDS-PAGE Rhodococcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.14.21 DBT monooxygenase
-
Rhodococcus sp.
1.14.14.21 dibenzothiophene monooxygenase
-
Rhodococcus sp.
1.14.14.21 dszC
-
Rhodococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.21 30
-
-
Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.21 7
-
-
Rhodococcus sp.

General Information

EC Number General Information Comment Organism
1.14.14.21 metabolism dibenzothiophene monooxygenase is the first enzyme involved in the degradation of dibenzothiophene Rhodococcus sp.