Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Konishi, J.; Maruhashi, K.
    Residue 345 of dibenzothiophene (DBT) sulfone monooxygenase is involved in C-S bond cleavage specificity of alkylated DBT sulfones (2003), Biotechnol. Lett., 25, 1199-1202.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.22 gene dszA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109 Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.22 L346A site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme Rhodococcus erythropolis
1.14.14.22 Q345A site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone Rhodococcus erythropolis
1.14.14.22 T344A site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme Rhodococcus erythropolis
1.14.14.22 T344A/Q345A site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Rhodococcus erythropolis
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Rhodococcus erythropolis IGTS8 / ATCC 53968
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.22 Rhodococcus erythropolis
-
 gene dszA
-
1.14.14.22 Rhodococcus erythropolis IGTS8 / ATCC 53968
-
 gene dszA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.22 3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 activity of mutant Q345A Rhodococcus erythropolis ? + 2 FMN + H2O
-
 ?
1.14.14.22 3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 activity of mutant Q345A Rhodococcus erythropolis IGTS8 / ATCC 53968 ? + 2 FMN + H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Rhodococcus erythropolis 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Rhodococcus erythropolis IGTS8 / ATCC 53968 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 additional information no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes Rhodococcus erythropolis ?
-
 ?
1.14.14.22 additional information no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes Rhodococcus erythropolis IGTS8 / ATCC 53968 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.22 DBT sulfone monooxygenase
-
 Rhodococcus erythropolis
1.14.14.22 dibenzothiophene sulfone monooxygenase
-
 Rhodococcus erythropolis
1.14.14.22 dszA
-
 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.22 FMNH2
-
 Rhodococcus erythropolis

General Information

EC Number General Information Comment Organism
1.14.14.22 additional information residue Q345 is involved in C-S bond cleavage specifically for alkylated dibenzothiophene sulfone Rhodococcus erythropolis