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Literature summary extracted from

  • Hao, N.; Mu, J.; Hu, N.; Xu, S.; Shen, P.; Yan, M.; Li, Y.; Xu, L.
    Implication of ornithine acetyltransferase activity on L-ornithine production in Corynebacterium glutamicum (2016), Biotechnol. Appl. Biochem., 63, 15-21.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.35 gene argJ, cloning in Escherichia coli strain DH5alpha, recombinant overexpression in Corynebacterium glutamicum strain 1006 Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.35 additional information inactivation of regulatory repressor argR gene and overexpression of argJ gene, argJ overexpression leads to decreased ornithine acetyltransferase activity due to product inhibition by L-ornithine Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.35 L-ornithine product inhibition Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Corynebacterium glutamicum
-
 L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate Corynebacterium glutamicum ATCC 13032
-
 L-ornithine + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.35 Corynebacterium glutamicum Q59280 gene argJ
-
2.3.1.35 Corynebacterium glutamicum ATCC 13032 Q59280 gene argJ
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.35 0.004
-
 enzyme activity in argJ-overexpressing strain 1006, pH 7.5, 30°C Corynebacterium glutamicum
2.3.1.35 0.008
-
 enzyme activity in mutant strain 1006DELTAargR, pH 7.5, 30°C Corynebacterium glutamicum
2.3.1.35 0.015
-
 enzyme activity in mutant strain 1006DELTAargR-DELTAargJ, pH 7.5, 30°C Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Corynebacterium glutamicum L-ornithine + N-acetyl-L-glutamate
-
 ?
2.3.1.35 N2-acetyl-L-ornithine + L-glutamate
-
 Corynebacterium glutamicum ATCC 13032 L-ornithine + N-acetyl-L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.35 argJ
-
 Corynebacterium glutamicum
2.3.1.35 OATase
-
 Corynebacterium glutamicum
2.3.1.35 ornithine acetyltransferase
-
 Corynebacterium glutamicum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.35 5
-
 pH 7.5, 30°C Corynebacterium glutamicum L-ornithine