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Literature summary extracted from
- Purification, characterization and crystallization of enzymes for dibenzothiophene desulfurization (2000), Bioseparation, 9, 185-188.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | additional information | flavin reductase (DszD) is essential for the enzyme activity of DszC | Rhodococcus erythropolis | |
| 1.14.14.22 | additional information | flavin reductase (DszD) is essential for the enzyme activity of DszA | Rhodococcus erythropolis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | 4-chloromercuribenzoic acid | - |
Rhodococcus erythropolis |  |
| 1.14.14.21 | additional information | thiol reagents and metal chelators inhibit the enzyme activity | Rhodococcus erythropolis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | additional information | a metal ion might be involved in DszC activity | Rhodococcus erythropolis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 45000 | - |
6 * 45000, SDS-PAGE | Rhodococcus erythropolis |
| 1.14.14.21 | 250000 | - |
gel filtration | Rhodococcus erythropolis |
| 1.14.14.22 | 50000 | - |
2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
| 1.14.14.22 | 97000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis D-1 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.21 | Rhodococcus erythropolis | A0A0C6DRW4 | gene dszC | - |
| 1.14.14.21 | Rhodococcus erythropolis D-1 | A0A0C6DRW4 | gene dszC | - |
| 1.14.14.22 | Rhodococcus erythropolis | Q6WE15 | gene dszA | - |
| 1.14.14.22 | Rhodococcus erythropolis D-1 | Q6WE15 | gene dszA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.21 | native enzyme 24fold to homogeneity from strain D-1 by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, and another different step of anion exchange chromatography, followed by gel filtration | Rhodococcus erythropolis |
| 1.14.14.22 | native enzyme 102fold to homogeneity from strain D-1 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 133 | - |
purified enzyme, pH 7.0, 30°C | Rhodococcus erythropolis |
| 1.14.14.22 | 807 | - |
purified enzyme, pH 7.0, 35°C | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 129% of the activity with dibenzothiophene | Rhodococcus erythropolis | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 129% of the activity with dibenzothiophene | Rhodococcus erythropolis D-1 | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 52% of the activity with dibenzothiophene | Rhodococcus erythropolis | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | 52% of the activity with dibenzothiophene | Rhodococcus erythropolis D-1 | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Rhodococcus erythropolis D-1 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | additional information | no or poor activity with 3,4-benzo-dibenzothiophene, biphenyl, carbazole, dibenzofuran, and fluorene, substrate specificity, overview. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszC | Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.21 | additional information | no or poor activity with 3,4-benzo-dibenzothiophene, biphenyl, carbazole, dibenzofuran, and fluorene, substrate specificity, overview. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszC | Rhodococcus erythropolis D-1 | ? | - |
? | |
| 1.14.14.21 | thioxanthen-9-one + FMNH2 + O2 | 45% of the activity with dibenzothiophene | Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.21 | thioxanthen-9-one + FMNH2 + O2 | 45% of the activity with dibenzothiophene | Rhodococcus erythropolis D-1 | ? | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszA | Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.22 | additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone. Interaction with purified flavin reductase from the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, with DszA | Rhodococcus erythropolis D-1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | homohexamer | 6 * 45000, SDS-PAGE | Rhodococcus erythropolis |
| 1.14.14.22 | homodimer | 2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | DBT monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.21 | dszC | - |
Rhodococcus erythropolis |
| 1.14.14.22 | DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.22 | dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 30 | - |
assay at | Rhodococcus erythropolis |
| 1.14.14.22 | 35 | - |
assay at | Rhodococcus erythropolis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 7 | - |
assay at | Rhodococcus erythropolis |
| 1.14.14.22 | 7 | - |
assay at | Rhodococcus erythropolis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | FMNH2 | - |
Rhodococcus erythropolis | |
| 1.14.14.22 | FMNH2 | - |
Rhodococcus erythropolis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | additional information | flavin reductase (DszD) is essential for the enzyme activity of DszC | Rhodococcus erythropolis |
| 1.14.14.22 | additional information | flavin reductase (DszD) is essential for the enzyme activity of DszA | Rhodococcus erythropolis |
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