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Literature summary extracted from

  • Sheng, W.; Yamashita, S.; Ohta, A.; Horiuchi, H.
    Functional differentiation of chitin synthases in Yarrowia lipolytica (2013), Biosci. Biotechnol. Biochem., 77, 1275-1281.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.16 genes CHS1-4, phylogenetic tree, expression of deeltion mutants, quantitative expression analysis by RT-PCR Yarrowia lipolytica

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.16 additional information construction of deletion mutants of the 4 isozymes CHS1-CHS4, phenotypes, overview Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Yarrowia lipolytica
-
 4 isozymes, CHS1-CHS4
-
2.4.1.16 Yarrowia lipolytica CXAU1
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 4 isozymes, CHS1-CHS4
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.16 cell culture growth of wild-type and enzyme mutant cells, overview Yarrowia lipolytica
-

General Information

EC Number General Information Comment Organism
2.4.1.16 malfunction the chs2DELTA mutant forms chained cells in which daughter cells are connected with mother cells and have abnormally thick septa at the bud neck. The chs4DELTA mutant shows remarkably reduced chitin content in its cell wall. The chs2DELTA, csm1DELTA, and csm2DELTA mutants are highly sensitive to chitin binding dyes, calcofluor white and Congo red, whereas the chs4DELTA mutant is resistant to calcofluor white. The lengths of the cellular long and short axes of populations of filamentous cells are decreased in the chs3DELTA mutant Yarrowia lipolytica
2.4.1.16 additional information chitin content in and morphological features of wild-type and enzyme mutant cells, overview Yarrowia lipolytica
2.4.1.16 physiological function isozymes Chs2 and Chs4 may play major roles in septum formation and cell wall chitin synthesis respectively, whereas isozymes Csm1 and Csm2 are involved in the maintenance of cell wall architecture and/or cell wall integrity. Isozyme Chs3 seems to be involved in cellular morphogenesis Yarrowia lipolytica