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Literature summary extracted from

  • da Silva Neto, A.M.; Torini de Souza, J.R.; Romanello, L.; Cassago, A.; Serrao, V.H.; DeMarco, R.; Brandao-Neto, J.; Garratt, R.C.; Pereira, H.D.
    Analysis of two Schistosoma mansoni uridine phosphorylases isoforms suggests the emergence of a protein with a non-canonical function (2016), Biochimie, 125, 12-22.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3 expressed in Escherichia coli BL21 Codon Plus cells Schistosoma mansoni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 free isoform UPa and in complex with thymidine, uracil, thymine or 5-fluorouracil, sitting drop vapor diffusion method, using 100 mM trisodium citrate pH5.6, 16% (w/v) PEG4000 and 17.5% (v/v) isopropanol or 100 mM bis-Tris pH 5.5, 25% (w/v) PEG3350 and 200 mM ammonium sulfate Schistosoma mansoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 0.031
-
 uridine pH and temperature not specified in the publication Schistosoma mansoni
2.4.2.3 0.0489
-
 thymidine pH and temperature not specified in the publication Schistosoma mansoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Schistosoma mansoni
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Schistosoma mansoni G4VGH9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3 Co-NTA agarose column chromatography Schistosoma mansoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 thymidine + phosphate
-
 Schistosoma mansoni thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Schistosoma mansoni uracil + alpha-D-ribose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.3 uPA isoform Schistosoma mansoni
2.4.2.3 UPb inactive isoform Schistosoma mansoni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.3 0.41
-
 thymidine pH and temperature not specified in the publication Schistosoma mansoni
2.4.2.3 35.9
-
 uridine pH and temperature not specified in the publication Schistosoma mansoni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.3 8.38
-
 thymidine pH and temperature not specified in the publication Schistosoma mansoni
2.4.2.3 1158
-
 uridine pH and temperature not specified in the publication Schistosoma mansoni