EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 86400 | - |
x * 90000, SDS-PAGE, x * 86400, calculated | Azotobacter chroococcum |
2.4.1.B34 | 90000 | - |
x * 90000, SDS-PAGE, x * 86400, calculated | Azotobacter chroococcum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.B34 | Azotobacter chroococcum | A0A0C4WTK3 | - |
- |
2.4.1.B34 | Azotobacter chroococcum NCIMB 8003 | A0A0C4WTK3 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 6.6 | - |
pH 6.5, 50°C | Azotobacter chroococcum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.B34 | amylose V | - |
Azotobacter chroococcum | ? | GtfB produces a high molecular mass polymer with a molecular mass about 80 times greater than that of the starting amylose V | ? | |
2.4.1.B34 | amylose V + maltose | - |
Azotobacter chroococcum | maltotriose + maltotetraose + ? | - |
? | |
2.4.1.B34 | Maltoheptaose | - |
Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | Maltoheptaose | - |
Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
2.4.1.B34 | Maltohexaose | - |
Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | Maltohexaose | - |
Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
2.4.1.B34 | maltopentaose | - |
Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | maltose | - |
Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | Maltotetraose | - |
Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | Maltotetraose | - |
Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
2.4.1.B34 | maltotriose | - |
Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | additional information | GtfD shows clear hydrolase/transglycosylase activity with malto-oligosaccharides with degree of polymerzation of 3 to 7 and forms a range of shorter and longer oligosaccharides. The enzyme is unable to synthesize consecutive alpha1->6 glucosidic bonds. Instead, it forms a high molecular mass and branched alpha-glucan with alternating alpha1->4 and alpha1->6 linkages from amylose/starch | Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | additional information | inactive on sucrose, panose, nigerose, beta-cyclodextrins, and isomalto-oligosaccharides with degree of polymerzation of 2, 3, and 5 | Azotobacter chroococcum | ? | - |
? | |
2.4.1.B34 | additional information | GtfD shows clear hydrolase/transglycosylase activity with malto-oligosaccharides with degree of polymerzation of 3 to 7 and forms a range of shorter and longer oligosaccharides. The enzyme is unable to synthesize consecutive alpha1->6 glucosidic bonds. Instead, it forms a high molecular mass and branched alpha-glucan with alternating alpha1->4 and alpha1->6 linkages from amylose/starch | Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
2.4.1.B34 | additional information | inactive on sucrose, panose, nigerose, beta-cyclodextrins, and isomalto-oligosaccharides with degree of polymerzation of 2, 3, and 5 | Azotobacter chroococcum NCIMB 8003 | ? | - |
? | |
2.4.1.B34 | potato starch | - |
Azotobacter chroococcum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.B34 | ? | x * 90000, SDS-PAGE, x * 86400, calculated | Azotobacter chroococcum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.B34 | Achr_35950 | - |
Azotobacter chroococcum |
2.4.1.B34 | GtfD | - |
Azotobacter chroococcum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 60 | - |
- |
Azotobacter chroococcum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 60 | - |
stable for at least 10 min | Azotobacter chroococcum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 6.5 | - |
- |
Azotobacter chroococcum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.B34 | 5.5 | 7 | more than 70% of maximum activity | Azotobacter chroococcum |