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Literature summary extracted from

  • Violante, S.; Ijlst, L.; Ruiter, J.; Koster, J.; van Lenthe, H.; Duran, M.; de Almeida, I.T.; Wanders, R.J.; Houten, S.M.; Ventura, F.V.
    Substrate specificity of human carnitine acetyltransferase: Implications for fatty acid and branched-chain amino acid metabolism (2013), Biochim. Biophys. Acta, 1832, 773-779.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.7 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.7 0.078
-
 acetyl-CoA peroxisomal enzyme, at pH 7.4 and 37°C Homo sapiens
2.3.1.7 0.24
-
 acetyl-CoA mitochondrial enzyme, at pH 7.4 and 37°C Homo sapiens
2.3.1.7 0.499
-
 butyryl-CoA mitochondrial enzyme, at pH 7.4 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.7 mitochondrion
-
 Homo sapiens 5739
-
2.3.1.7 peroxisome
-
 Homo sapiens 5777
-
2.3.1.21 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.21 additional information Homo sapiens the enzyme is inactive with short-chain acyl-CoAs and intermediates of the branched-chain amino acid oxidation pathway ?
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine Homo sapiens
-
 CoA + L-palmitoylcarnitine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.7 Homo sapiens
-
-
-
2.3.1.21 Homo sapiens P23786
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.7 HisLink protein purification resin column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.7 acetyl-CoA + L-carnitine
-
 Homo sapiens CoA + O-acetylcarnitine
-
 ?
2.3.1.7 butyryl-CoA + L-carnitine
-
 Homo sapiens CoA + O-butyrylcarnitine
-
 ?
2.3.1.7 additional information the enzyme shows some activity with acyl-CoAs of longer chain length including C12- and C14-CoA, but not with C16-CoA. Trans-2-enoyl-CoA intermediates such as trans-2-ene-C4-CoA (crotonyl-CoA, trans-2-C4:1-CoA), trans-2-ene-3-methyl-C4-CoA (3-methylcrotonyl-CoA), and trans-2-ene-2-methyl-C4-CoA are poor substrates for the enzyme and no activity is found with dicarboxylic acyl-CoA esters Homo sapiens ?
-
 ?
2.3.1.7 octanoyl-CoA + L-carnitine
-
 Homo sapiens CoA + O-octanoylcarnitine
-
 ?
2.3.1.7 palmitoyl-CoA + L-carnitine
-
 Homo sapiens CoA + O-palmitoylcarnitine
-
 ?
2.3.1.7 propionyl-CoA + L-carnitine
-
 Homo sapiens CoA + O-propionylcarnitine
-
 ?
2.3.1.21 4,8-dimethylnonanoyl-CoA + L-carnitine
-
 Homo sapiens ?
-
 ?
2.3.1.21 additional information the enzyme is inactive with short-chain acyl-CoAs and intermediates of the branched-chain amino acid oxidation pathway Homo sapiens ?
-
 ?
2.3.1.21 additional information no activity with 2,6-dimethylheptanoyl-CoA Homo sapiens ?
-
 ?
2.3.1.21 palmitoyl-CoA + L-carnitine
-
 Homo sapiens CoA + L-palmitoylcarnitine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.7 carnitine acetyltransferase
-
 Homo sapiens
2.3.1.7 CRAT
-
 Homo sapiens
2.3.1.21 carnitine palmitoyltransferase 2
-
 Homo sapiens
2.3.1.21 CPT2
-
 Homo sapiens