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Literature summary extracted from

  • Blamey, J.M.; Adams, M.W.
    Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima (1994), Biochemistry, 33, 1000-1007.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.1 thiamine diphosphate stimulatory Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.1 additional information not inhibitory: carbon monoxide, sodium cyanide, sodium fluoride, sodium azide Thermotoga maritima
1.2.7.1 Sodium nitrite
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.34
-
 CoA pH 8.2, 80°C Thermotoga maritima
1.2.7.1 14.5
-
 pyruvate pH 8.2, 80°C Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 13000
-
 x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 23000
-
 x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 34000
-
 x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 43000
-
 x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 155000
-
 gel fitlration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Thermotoga maritima
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 25°C, half-life in presence of air 70 min Thermotoga maritima

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1 purification under strictly unaerobic conditions Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 additional information CoA is absolutely required. No substrates: 2-oxoglutarate, phenyl pyruvate, or indolyl pyruvate Thermotoga maritima ?
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen
-
 Thermotoga maritima acetyl-CoA + CO2 + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 multimer x * 43000, plus x * 34000, plus x * 23000, plus x * 13000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 90
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.1 80
-
 half-life 15 h Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.1 [4Fe-4S]-center proteins contains at least two ferredoxin-type [4Fe-4S] clusters per molecule. Km value for ferredoxin is 0.026 microM Thermotoga maritima

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.7.1 54
-
 Sodium nitrite pH 8.2, 80°C Thermotoga maritima