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Literature summary extracted from

  • Kanchan, K.; Erguelen, E.; Kiraly, R.; Simon-Vecsei, Z.; Fuxreiter, M.; Fesues, L.
    Identification of a specific one amino acid change in recombinant human transglutaminase 2 that regulates its activity and calcium sensitivity (2013), Biochem. J., 455, 261-272.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Escherichia coli Rosetta 2 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 G224V the mutation increases the enzyme's calcium-binding affinity and transamidation activity 10fold and isopeptidase activity severalfold Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 1.9
-
 alpha2 plasmin inhibitor at pH 7.5 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 [protein]-L-glutamine + alkylamine Homo sapiens
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Homo sapiens P21980
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 2-aminobenzoyl-APE(gamma-Cad-2,4-dinitrophenyl)QEA + Gly-OMe
-
 Homo sapiens ?
-
 ?
2.3.2.13 5-(biotinamido)pentylamine + N,N-dimethylated casein
-
 Homo sapiens ? + NH3
-
 ?
2.3.2.13 alpha2 plasmin inhibitor + ?
-
 Homo sapiens ?
-
 ?
2.3.2.13 [protein]-L-glutamine + alkylamine
-
 Homo sapiens [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TG2
-
 Homo sapiens
2.3.2.13 transglutaminase 2
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.13 45
-
 melting temperature Homo sapiens