Literature summary extracted from

But, S.Y.; Khmelenina, V.N.; Reshetnikov, A.S.; Mustakhimov, I.I.; Kalyuzhnaya, M.G.; Trotsenko, Y.A.
Sucrose metabolism in halotolerant methanotroph Methylomicrobium alcaliphilum 20Z (2015), Arch. Microbiol., 197, 471-480.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.1.4	gene ams, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the His6-tagged in Escherichia coli strain Rosettta (DE3)	Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.1.4	additional information	-	additional information	classical Michaelis-Menten kinetics in the reactions of sucrose hydrolysis and transglycosylation	Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.1.4	32700	-	x * 32700, SDS-PAGE	Methylotuvimicrobium alcaliphilum

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.1.4	sucrose + [(1->4)-alpha-D-glucosyl]n	Methylotuvimicrobium alcaliphilum	-	D-fructose + [(1->4)-alpha-D-glucosyl]n+1	-	?
2.4.1.4	sucrose + [(1->4)-alpha-D-glucosyl]n	Methylotuvimicrobium alcaliphilum 20Z	-	D-fructose + [(1->4)-alpha-D-glucosyl]n+1	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.4	Methylotuvimicrobium alcaliphilum	G4T024	gene ams	-
2.4.1.4	Methylotuvimicrobium alcaliphilum 20Z	G4T024	gene ams	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.4	sucrose + [(1->4)-alpha-D-glucosyl]n	-	Methylotuvimicrobium alcaliphilum	D-fructose + [(1->4)-alpha-D-glucosyl]n+1	-	?
2.4.1.4	sucrose + [(1->4)-alpha-D-glucosyl]n	-	Methylotuvimicrobium alcaliphilum 20Z	D-fructose + [(1->4)-alpha-D-glucosyl]n+1	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.4.1.4	?	x * 32700, SDS-PAGE	Methylotuvimicrobium alcaliphilum

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.1.4	AMS	-	Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.4.1.4	30	-	assay at	Methylotuvimicrobium alcaliphilum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.4.1.4	7	-	assay at	Methylotuvimicrobium alcaliphilum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)