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Literature summary extracted from

  • Inokuma, K.; Nakashimada, Y.; Akahoshi, T.; Nishio, N.
    Characterization of enzymes involved in the ethanol production of Moorella sp. HUC22-1 (2007), Arch. Microbiol., 188, 37-45.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.2 synthesis 50 microg of alcohol dehydrogenase AdhA, EC 1.1.1.2, and 50 microg actaldehyde dehydrogenase AldH, EC 1.2.1.10,in buffer solution (pH 8.0) containing NADPH, NADH and acetyl-CoA at 60°C, produce 1.6 mM ethanol from 3 mM acetyl-CoA after 90 min Moorella sp. HUC22-1
1.2.1.10 synthesis 50 microg of alcohol dehydrogenase AdhA, EC 1.1.1.2, and 50 microg actaldehyde dehydrogenase AldH, EC 1.2.1.10, in buffer solution (pH 8.0) containing NADPH, NADH and acetyl-CoA at 60°C, produce 1.6 mM ethanol from 3 mM acetyl-CoA after 90 min Moorella sp. HUC22-1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 expression in Escherichia coli Moorella sp. HUC22-1
1.2.1.10 expression in Escherichia coli Moorella sp. HUC22-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 10
-
 acetaldehyde isoform AdhA, pH 8.0, 60°C Moorella sp. HUC22-1
1.1.1.2 40
-
 ethanol isoform AdhA, pH 8.0, 60°C Moorella sp. HUC22-1
1.1.1.2 55.8
-
 acetaldehyde isoform AdhB, pH 8.0, 60°C Moorella sp. HUC22-1
1.2.1.10 1.6
-
 acetyl-CoA pH 8.0, 60°C Moorella sp. HUC22-1
1.2.1.10 4.3
-
 acetaldehyde pH 8.0, 60°C Moorella sp. HUC22-1

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Moorella sp. HUC22-1
-
-
-
1.2.1.10 Moorella sp. HUC22-1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 0.032
-
 isoform AdhA, oxidation of ethanol, cofactor NAD+, pH 8.0, 60°C Moorella sp. HUC22-1
1.1.1.2 0.049
-
 isoform AdhA, reduction of acetaldehyde, cofactor NADH, pH 8.0, 60°C Moorella sp. HUC22-1
1.1.1.2 2.9
-
 isoform AdhB, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60°C Moorella sp. HUC22-1
1.1.1.2 11.9
-
 isoform AdhA, oxidation of ethanol, cofactor NADP+, pH 8.0, 60°C Moorella sp. HUC22-1
1.1.1.2 50.4
-
 isoform AdhA, reduction of acetaldehyde, cofactor NADPH, pH 8.0, 60°C Moorella sp. HUC22-1
1.2.1.10 2.8
-
 cleavage of thioester, cofactor NADPH, pH 8.0, 60°C Moorella sp. HUC22-1
1.2.1.10 3.7
-
 condensation of thioester, cofactor NADP+, pH 10.0, 60°C Moorella sp. HUC22-1
1.2.1.10 18.8
-
 cleavage of thioester, cofactor NADH, pH 8.0, 60°C Moorella sp. HUC22-1
1.2.1.10 29.9
-
 condensation of thioester, cofactor NAD+, pH 10.0, 60°C Moorella sp. HUC22-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 acetaldehyde + NADPH + H+
-
 Moorella sp. HUC22-1 ethanol + NADP+
-
 r
1.1.1.2 butan-1-ol + NADP+ isoform AdhA, 238% of the activity with acetaldehyde Moorella sp. HUC22-1 butanal + NADPH + H+
-
 r
1.1.1.2 butanal + NADPH + H+ isoform AdhA, 625%, isoform AdhB, 108% of the activity with acetaldehyde Moorella sp. HUC22-1 butan-1-ol + NADP+
-
 r
1.1.1.2 ethanol + NADP+ isoform AdhB: no oxidation of ethanol Moorella sp. HUC22-1 acetaldehyde + NADPH + H+
-
 r
1.1.1.2 isobutanol + NADP+ isoform AdhA, 221% of the activity with acetaldehyde Moorella sp. HUC22-1 isobutyl aldehyde + NADPH + H+
-
 r
1.1.1.2 isobutyl aldehyde + NADPH + H+ isoform AdhA, 469%, isoform AdhB, 48% of the activity with acetaldehyde Moorella sp. HUC22-1 isobutanol + NADP+
-
 r
1.1.1.2 propan-1-ol + NADP+ isoform AdhA, 162% of the activity with acetaldehyde Moorella sp. HUC22-1 propanal + NADPH + H+
-
 r
1.1.1.2 propanal + NADPH + H+ isoform AdhA, 50%, isoform AdhB, 62% of the activity with acetaldehyde Moorella sp. HUC22-1 propan-1-ol + NADP+
-
 r
1.2.1.10 acetaldehyde + NAD+ + CoA
-
 Moorella sp. HUC22-1 acetyl-CoA + NADH + H+
-
 r
1.2.1.10 acetaldehyde + NADP+ + CoA
-
 Moorella sp. HUC22-1 acetyl-CoA + NADPH + H+
-
 r
1.2.1.10 acetyl-CoA + NADH + H+
-
 Moorella sp. HUC22-1 acetaldehyde + NAD+ + CoA
-
 r
1.2.1.10 acetyl-CoA + NADPH + H+
-
 Moorella sp. HUC22-1 acetaldehyde + NADP+ + CoA
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 AdhA
-
 Moorella sp. HUC22-1
1.1.1.2 AdhB
-
 Moorella sp. HUC22-1
1.2.1.10 ALDH
-
 Moorella sp. HUC22-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 60
-
 at pH 8.0 Moorella sp. HUC22-1
1.2.1.10 60
-
 at pH 8.0 Moorella sp. HUC22-1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.2 50
-
 isoform AdhA, 84%, isoform AdhB, 26% of maximum activity Moorella sp. HUC22-1
1.1.1.2 70
-
 isoform AdhA, 61%, isoform AdhB 64% of maximum activity Moorella sp. HUC22-1
1.2.1.10 50
-
 71% of maximum activity Moorella sp. HUC22-1
1.2.1.10 70
-
 52% of maximum activity Moorella sp. HUC22-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 8
-
 reduction of acetaldehyde Moorella sp. HUC22-1
1.1.1.2 10
-
 oxidation of ethanol Moorella sp. HUC22-1
1.2.1.10 8
-
 cleavage of thioester Moorella sp. HUC22-1
1.2.1.10 10
-
 condensation of thiopester Moorella sp. HUC22-1

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 additional information isoform AdhA, less than 1% activity with NAD(H), isoform AdhB, no activity with NAD(H) Moorella sp. HUC22-1
1.1.1.2 NADPH
-
 Moorella sp. HUC22-1
1.2.1.10 NADH NADH is preferred 7-8fold over NADPH Moorella sp. HUC22-1
1.2.1.10 NADPH NADH is preferred 7-8fold over NADPH Moorella sp. HUC22-1