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Literature summary extracted from

  • Diricks, M.; De Bruyn, F.; Van Daele, P.; Walmagh, M.; Desmet, T.
    Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes (2015), Appl. Microbiol. Biotechnol., 99, 8465-8474.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.13 gene SuSyAc, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Acidithiobacillus caldus
2.4.1.13 gene SuSyDa, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Denitrovibrio acetiphilus
2.4.1.13 gene SuSyMr, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Melioribacter roseus
2.4.1.13 gene SuSyNe, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Nitrosomonas europaea

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.13 A642N site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme Acidithiobacillus caldus
2.4.1.13 L636Q/A642N site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme Acidithiobacillus caldus
2.4.1.13 L636Q/V641R/A642N site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme Acidithiobacillus caldus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.13 GDP substrate inhibition occurs in the presence of GDP at concentrations above 10 mM Nitrosomonas europaea
2.4.1.13 Mg2+
-
 Denitrovibrio acetiphilus
2.4.1.13 Mg2+
-
 Melioribacter roseus
2.4.1.13 Mg2+
-
 Nitrosomonas europaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 additional information
-
 additional information Michaelis-Menten kinetics Denitrovibrio acetiphilus
2.4.1.13 additional information
-
 additional information Michaelis-Menten kinetics Melioribacter roseus
2.4.1.13 additional information
-
 additional information Michaelis-Menten kinetics Acidithiobacillus caldus
2.4.1.13 additional information
-
 additional information Michaelis-Menten kinetics, except for GDP Nitrosomonas europaea
2.4.1.13 0.17
-
 ADP pH 7.0, 60°C Acidithiobacillus caldus
2.4.1.13 0.44
-
 ADP pH 7.0, 60°C Nitrosomonas europaea
2.4.1.13 0.69
-
 UDP pH 7.0, 60°C Nitrosomonas europaea
2.4.1.13 1.28
-
 CDP pH 7.0, 60°C Nitrosomonas europaea
2.4.1.13 1.56
-
 GDP pH 7.0, 60°C Nitrosomonas europaea
2.4.1.13 7.8
-
 UDP pH 7.0, 60°C Acidithiobacillus caldus
2.4.1.13 8.5
-
 GDP pH 7.0, 60°C Acidithiobacillus caldus
2.4.1.13 16.9
-
 CDP pH 7.0, 60°C Acidithiobacillus caldus
2.4.1.13 140
-
 sucrose pH 7.0, 60°C, with ADP Nitrosomonas europaea
2.4.1.13 321
-
 sucrose pH 7.0, 60°C, with UDP Nitrosomonas europaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 Mg2+ slightly stimulating Acidithiobacillus caldus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.13 NDP-glucose + D-fructose Denitrovibrio acetiphilus
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose Melioribacter roseus
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose Nitrosomonas europaea
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose Acidithiobacillus caldus
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose Denitrovibrio acetiphilus DSM 12809
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose Melioribacter roseus JCM 17771
-
 NDP + sucrose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Acidithiobacillus caldus A0A059ZV61
-
-
2.4.1.13 Acidithiobacillus caldus ATCC 51756 A0A059ZV61
-
-
2.4.1.13 Denitrovibrio acetiphilus D4H6M0
-
-
2.4.1.13 Denitrovibrio acetiphilus DSM 12809 D4H6M0
-
-
2.4.1.13 Melioribacter roseus I7A3T6
-
-
2.4.1.13 Melioribacter roseus JCM 17771 I7A3T6
-
-
2.4.1.13 Nitrosomonas europaea Q820M5
-
-
2.4.1.13 Nitrosomonas europaea ATCC 19718 Q820M5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Denitrovibrio acetiphilus
2.4.1.13 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Melioribacter roseus
2.4.1.13 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Nitrosomonas europaea
2.4.1.13 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Acidithiobacillus caldus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.13 additional information optimal growth at 20-30°C Nitrosomonas europaea
-
2.4.1.13 additional information optimal growth at 35-37°C Denitrovibrio acetiphilus
-
2.4.1.13 additional information optimal growth at 45°C Acidithiobacillus caldus
-
2.4.1.13 additional information optimal growth at 52-55°C Melioribacter roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 ADP-glucose + D-fructose
-
 Denitrovibrio acetiphilus ADP + sucrose
-
 r
2.4.1.13 ADP-glucose + D-fructose
-
 Melioribacter roseus ADP + sucrose
-
 r
2.4.1.13 ADP-glucose + D-fructose
-
 Nitrosomonas europaea ADP + sucrose
-
 r
2.4.1.13 ADP-glucose + D-fructose
-
 Acidithiobacillus caldus ADP + sucrose
-
 r
2.4.1.13 ADP-glucose + D-fructose
-
 Nitrosomonas europaea ATCC 19718 ADP + sucrose
-
 r
2.4.1.13 ADP-glucose + D-fructose
-
 Denitrovibrio acetiphilus DSM 12809 ADP + sucrose
-
 r
2.4.1.13 ADP-glucose + D-fructose
-
 Melioribacter roseus JCM 17771 ADP + sucrose
-
 r
2.4.1.13 ADP-glucose + D-fructose
-
 Acidithiobacillus caldus ATCC 51756 ADP + sucrose
-
 r
2.4.1.13 CDP-glucose + D-fructose
-
 Nitrosomonas europaea CDP + sucrose
-
 r
2.4.1.13 CDP-glucose + D-fructose
-
 Acidithiobacillus caldus CDP + sucrose
-
 r
2.4.1.13 CDP-glucose + D-fructose
-
 Nitrosomonas europaea ATCC 19718 CDP + sucrose
-
 r
2.4.1.13 CDP-glucose + D-fructose
-
 Acidithiobacillus caldus ATCC 51756 CDP + sucrose
-
 r
2.4.1.13 GDP-glucose + D-fructose
-
 Nitrosomonas europaea GDP + sucrose
-
 r
2.4.1.13 GDP-glucose + D-fructose
-
 Acidithiobacillus caldus GDP + sucrose
-
 r
2.4.1.13 GDP-glucose + D-fructose
-
 Nitrosomonas europaea ATCC 19718 GDP + sucrose
-
 r
2.4.1.13 GDP-glucose + D-fructose
-
 Acidithiobacillus caldus ATCC 51756 GDP + sucrose
-
 r
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Denitrovibrio acetiphilus ?
-
 ?
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Melioribacter roseus ?
-
 ?
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Nitrosomonas europaea ?
-
 ?
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Acidithiobacillus caldus ?
-
 ?
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Nitrosomonas europaea ATCC 19718 ?
-
 ?
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Denitrovibrio acetiphilus DSM 12809 ?
-
 ?
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Melioribacter roseus JCM 17771 ?
-
 ?
2.4.1.13 additional information UDP-glucose is preferred before ADP-glucose Acidithiobacillus caldus ATCC 51756 ?
-
 ?
2.4.1.13 NDP-glucose + D-fructose
-
 Denitrovibrio acetiphilus NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose
-
 Melioribacter roseus NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose
-
 Nitrosomonas europaea NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose
-
 Acidithiobacillus caldus NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose
-
 Denitrovibrio acetiphilus DSM 12809 NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose
-
 Melioribacter roseus JCM 17771 NDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Denitrovibrio acetiphilus UDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Melioribacter roseus UDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Nitrosomonas europaea UDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Acidithiobacillus caldus UDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Nitrosomonas europaea ATCC 19718 UDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Denitrovibrio acetiphilus DSM 12809 UDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Melioribacter roseus JCM 17771 UDP + sucrose
-
 r
2.4.1.13 UDP-glucose + D-fructose
-
 Acidithiobacillus caldus ATCC 51756 UDP + sucrose
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.13 More structure-function relationship analysis, overview Denitrovibrio acetiphilus
2.4.1.13 More structure-function relationship analysis, overview Melioribacter roseus
2.4.1.13 More structure-function relationship analysis, overview Nitrosomonas europaea
2.4.1.13 More structure-function relationship analysis, overview Acidithiobacillus caldus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.13 SuSy
-
 Denitrovibrio acetiphilus
2.4.1.13 SuSy
-
 Melioribacter roseus
2.4.1.13 SuSy
-
 Nitrosomonas europaea
2.4.1.13 SuSy
-
 Acidithiobacillus caldus
2.4.1.13 SuSyAc
-
 Acidithiobacillus caldus
2.4.1.13 SuSyDa
-
 Denitrovibrio acetiphilus
2.4.1.13 SuSyMr
-
 Melioribacter roseus
2.4.1.13 SuSyNe
-
 Nitrosomonas europaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.13 60
-
-
 Acidithiobacillus caldus
2.4.1.13 65
-
-
 Denitrovibrio acetiphilus
2.4.1.13 75
-
-
 Nitrosomonas europaea
2.4.1.13 80
-
-
 Melioribacter roseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.13 60
-
 purified recombinant His-tagged enzyme, without substrates, 15 min, 38% activity remaining Melioribacter roseus
2.4.1.13 60
-
 purified recombinant His-tagged enzyme, without substrates, 15 min, 54% activity remaining Nitrosomonas europaea
2.4.1.13 60
-
 purified recombinant His-tagged enzyme, without substrates, 15 min, 96% activity remaining Acidithiobacillus caldus
2.4.1.13 60
-
 purified recombinant His-tagged enzyme, without substrates, 15 min, no activity remaining Denitrovibrio acetiphilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 5
-
-
 Nitrosomonas europaea
2.4.1.13 5.5
-
-
 Acidithiobacillus caldus
2.4.1.13 6
-
-
 Denitrovibrio acetiphilus
2.4.1.13 7
-
-
 Melioribacter roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.13 5.5 7.5 over 40% of maximal enzyme activity within this range Denitrovibrio acetiphilus
2.4.1.13 5.5 7.5 over 40% of maximal enzyme activity within this range Melioribacter roseus
2.4.1.13 5.5 7.5 over 40% of maximal enzyme activity within this range Nitrosomonas europaea
2.4.1.13 5.5 7.5 over 40% of maximal enzyme activity within this range Acidithiobacillus caldus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.13 50
-
 GDP pH 7.0, 60°C Nitrosomonas europaea

General Information

EC Number General Information Comment Organism
2.4.1.13 additional information structure-function relationship analysis, overview Denitrovibrio acetiphilus
2.4.1.13 additional information structure-function relationship analysis, overview Melioribacter roseus
2.4.1.13 additional information structure-function relationship analysis, overview Nitrosomonas europaea
2.4.1.13 additional information structure-function relationship analysis, overview Acidithiobacillus caldus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.13 0.3
-
 sucrose pH 7.0, 60°C, with UDP Nitrosomonas europaea
2.4.1.13 1
-
 sucrose pH 7.0, 60°C, with ADP Nitrosomonas europaea
2.4.1.13 14
-
 CDP pH 7.0, 60°C Nitrosomonas europaea
2.4.1.13 39
-
 GDP pH 7.0, 60°C Nitrosomonas europaea
2.4.1.13 71
-
 ADP pH 7.0, 60°C Nitrosomonas europaea
2.4.1.13 150
-
 UDP pH 7.0, 60°C Nitrosomonas europaea