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Literature summary extracted from

  • Yao, S.; Mikkelsen, M.J.
    Metabolic engineering to improve ethanol production in Thermoanaerobacter mathranii (2010), Appl. Microbiol. Biotechnol., 88, 199-208.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.6 synthesis Thermoanaerobacter mathranii can produce ethanol from lignocellulosic biomass at high temperatures. Deletion of the Ldh gene coding for lactate dehydrogenase eliminates an NADH oxidation pathway. To further facilitate NADH regeneration used for ethanol formation, heterologous gene GldA is expressed leading to increased ethanol yield in the presence of glycerol using xylose as a substrate. The metabolism of the cells is shifted toward the production of ethanol over acetate, hence restoring the redox balance. The recombinant acquired the capability to utilize glycerol as an extra carbon source in the presence of xylose resulting in a higher ethanol yield Thermotoga maritima
1.1.1.27 synthesis Thermoanaerobacter mathranii can produce ethanol from lignocellulosic biomass at high temperatures. Deletion of the Ldh gene coding for lactate dehydrogenase eliminates an NADH oxidation pathway. To further facilitate NADH regeneration used for ethanol formation, a heterologous gene GldA encoding an NAD+-dependent glycerol dehydrogenase is expressed leading to increased ethanol yield in the presence of glycerol using xylose as a substrate. The metabolism of the cells is shifted toward the production of ethanol over acetate, hence restoring the redox balance. The recombinant enzyme acquired the capability to utilize glycerol as an extra carbon source in the presence of xylose resulting in a higher ethanol yield Thermoanaerobacter mathranii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Thermotoga maritima Q9WYQ4
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1.1.1.6 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 Q9WYQ4
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1.1.1.27 Thermoanaerobacter mathranii
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-
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1.1.1.27 Thermoanaerobacter mathranii BG1
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Synonyms

EC Number Synonyms Comment Organism
1.1.1.6 GldA
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Thermotoga maritima