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Literature summary extracted from

  • Guo, H.; Madzak, C.; Du, G.; Zhou, J.
    Mutagenesis of conserved active site residues of dihydrolipoamide succinyltransferase enhances the accumulation of alpha-ketoglutarate in Yarrowia lipolytica (2016), Appl. Microbiol. Biotechnol., 100, 649-659.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.61 ATP
-
 Yarrowia lipolytica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.61 subcloning in Escherichia coli strain JM109 Yarrowia lipolytica

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.61 D423A site-directed mutagenesis, the corresponding Km value of the D423A variant is 233% of the wild-type enzyme Km, while the kcat value of the variant is 4% compared to the wild-type enzyme Yarrowia lipolytica
2.3.1.61 D423E site-directed mutagenesis, the corresponding activity of the D423E variant is 30% of the wild-type activity Yarrowia lipolytica
2.3.1.61 D423G site-directed mutagenesis, the corresponding Km value of the D423S variant is 200% of the wild-type enzyme Km Yarrowia lipolytica
2.3.1.61 D423S site-directed mutagenesis, the corresponding Km value of the D423S variant is 161% of the wild-type enzyme Km, while the kcat value of the variant is 9% compared to the wild-type enzyme Yarrowia lipolytica
2.3.1.61 H419del site-directed mutagenesis, the mutant is almost inactive Yarrowia lipolytica
2.3.1.61 H419S site-directed mutagenesis, the mutant shows a the kcat value of 6% compared to the wild-type Yarrowia lipolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.61 additional information
-
 additional information Michaelis-Menten kinetics Yarrowia lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.61 mitochondrion
-
 Yarrowia lipolytica 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.61 Mg2+ required Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.61 Yarrowia lipolytica Q6C5L8
-
-
2.3.1.61 Yarrowia lipolytica E 150 Q6C5L8
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.61 dihydrolipoamide succinyltransferase
-
 Yarrowia lipolytica
2.3.1.61 DLST
-
 Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.61 30
-
 assay at Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.61 7.2
-
 assay at Yarrowia lipolytica

General Information

EC Number General Information Comment Organism
2.3.1.61 malfunction individual overexpression of dihydrolipoamide succinyltransferase, which serves as the inner core of KGDH, decreases overall activity of the enzyme complex, overexpression of mutated DLST not only impairs balanced assembly of KGDH, but also disrupts the catalytic integrity of the enzyme complex Yarrowia lipolytica
2.3.1.61 additional information the catalytic reaction of the enzyme depend on active site residues His419 and Asp423 Yarrowia lipolytica