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Literature summary extracted from

  • Gangoiti, J.; Pijning, T.; Dijkhuizen, L.
    The Exiguobacterium sibiricum 255-15 GtfC enzyme represents a novel glycoside hydrolase 70 subfamily of 4,6-alpha-glucanotransferase enzymes (2016), Appl. Environ. Microbiol., 82, 756-766.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.B34 Ca2+ 1 mM, slight activation Exiguobacterium sibiricum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B34 expression in Escherichia coli Exiguobacterium sibiricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.B34 99000
-
 x * 99000, calculated Exiguobacterium sibiricum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B34 Exiguobacterium sibiricum B1YMN6 enzyme GtfC differs in the domain order from dextransucrase and GtfB enzymes, and it completely lacks domain V
-
2.4.1.B34 Exiguobacterium sibiricum DSM 17290 B1YMN6 enzyme GtfC differs in the domain order from dextransucrase and GtfB enzymes, and it completely lacks domain V
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.B34 2.2
-
 40°C, pH 6.0 Exiguobacterium sibiricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B34 amylose V
-
 Exiguobacterium sibiricum ?
-
 ?
2.4.1.B34 amylose V
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
2.4.1.B34 maltoheptaose + H2O
-
 Exiguobacterium sibiricum D-glucose + maltotriose + maltohexaose first clear products of the reaction ?
2.4.1.B34 Maltohexaose
-
 Exiguobacterium sibiricum ?
-
 ?
2.4.1.B34 Maltohexaose
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
2.4.1.B34 maltopentaose
-
 Exiguobacterium sibiricum ?
-
 ?
2.4.1.B34 maltopentaose
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
2.4.1.B34 Maltotetraose
-
 Exiguobacterium sibiricum ?
-
 ?
2.4.1.B34 Maltotetraose
-
 Exiguobacterium sibiricum DSM 17290 ?
-
 ?
2.4.1.B34 additional information GtfC acts on maltooligosaccharides and amylose-V yielding linear gluco-oligomers also containing, besides alpha1->4, alpha1->6 glycosidic linkages. In the product mixture, free glucose units, 4-substituted reducing-end glucose residues and trace amounts of 6-substituted reducing-end glucose residues are present. The higher the degree of polymerization of the substrate, the higher the percentages of alpha1->6 glycosidic linkages introduced into the product. No substrates: maltose, maltotriose, sucrose, nigerose, isomaltooliogosaccharides, panose, reuteran Exiguobacterium sibiricum ?
-
 ?
2.4.1.B34 additional information GtfC acts on maltooligosaccharides and amylose-V yielding linear gluco-oligomers also containing, besides alpha1->4, alpha1->6 glycosidic linkages. In the product mixture, free glucose units, 4-substituted reducing-end glucose residues and trace amounts of 6-substituted reducing-end glucose residues are present. The higher the degree of polymerization of the substrate, the higher the percentages of alpha1->6 glycosidic linkages introduced into the product. No substrates: maltose, maltotriose, sucrose, nigerose, isomaltooliogosaccharides, panose, reuteran Exiguobacterium sibiricum DSM 17290 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B34 ? x * 99000, calculated Exiguobacterium sibiricum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B34 Exig_2648
-
 Exiguobacterium sibiricum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B34 45
-
-
 Exiguobacterium sibiricum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.B34 30
-
 30% of maximum activity Exiguobacterium sibiricum
2.4.1.B34 55
-
 drastic decrease above Exiguobacterium sibiricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B34 6
-
-
 Exiguobacterium sibiricum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.B34 4 7.5 more than 50% of maximum activity Exiguobacterium sibiricum