EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.32 | expressed in Escherichia coli BL21(DE3) cells | Rhodococcus jostii |
1.14.13.220 | expression in Escherichia coli BL21 | Rhodococcus jostii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.32 | 34000 | - |
x * 34000, SDS-PAGE | Rhodococcus jostii |
1.14.13.220 | 59000 | - |
1 * 59000, SDS-PAGE | Rhodococcus jostii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.32 | 2-maleylacetate + NADH + H+ | Rhodococcus jostii | - |
3-oxoadipate + NAD+ | - |
? | |
1.3.1.32 | 2-maleylacetate + NADPH + H+ | Rhodococcus jostii | - |
3-oxoadipate + NADP+ | - |
? | |
1.14.13.220 | resorcinol + NADH + H+ + O2 | Rhodococcus jostii | the enzyme gamma-resorcylate catabolic-pathway | hydroxyquinol + NAD+ + H2O | - |
? | |
4.1.1.103 | 2,6-dihydroxybenzoate | Rhodococcus jostii | - |
1,3-dihydroxybenzene + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.32 | Rhodococcus jostii | - |
- |
- |
1.14.13.220 | Rhodococcus jostii | - |
- |
- |
4.1.1.103 | Rhodococcus jostii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.220 | recombinant His-tagged protein | Rhodococcus jostii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.32 | 2-maleylacetate + NADH + H+ | - |
Rhodococcus jostii | 3-oxoadipate + NAD+ | - |
? | |
1.3.1.32 | 2-maleylacetate + NADPH + H+ | - |
Rhodococcus jostii | 3-oxoadipate + NADP+ | - |
? | |
1.14.13.220 | resorcinol + NADH + H+ + O2 | the enzyme gamma-resorcylate catabolic-pathway | Rhodococcus jostii | hydroxyquinol + NAD+ + H2O | - |
? | |
1.14.13.220 | resorcinol + NADH + H+ + O2 | resorcinol i.e. benzene-1,3-diol. The enzyme has no activity with NADPH | Rhodococcus jostii | hydroxyquinol + NAD+ + H2O | hydroxyquinol i.e. benzene-1,2,4-triol | ? | |
4.1.1.103 | 2,6-dihydroxybenzoate | - |
Rhodococcus jostii | 1,3-dihydroxybenzene + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.32 | ? | x * 34000, SDS-PAGE | Rhodococcus jostii |
1.14.13.220 | monomer | 1 * 59000, SDS-PAGE | Rhodococcus jostii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.32 | tsdD | - |
Rhodococcus jostii |
1.14.13.220 | tsdB | - |
Rhodococcus jostii |
4.1.1.103 | TsdA | - |
Rhodococcus jostii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.220 | 30 | - |
assay at | Rhodococcus jostii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.220 | 8 | - |
assay at | Rhodococcus jostii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.32 | NADH | - |
Rhodococcus jostii | |
1.3.1.32 | NADPH | - |
Rhodococcus jostii | |
1.14.13.220 | NADH | the enzyme has no activity with NADPH | Rhodococcus jostii |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.13.220 | Rhodococcus jostii | transcription of tsdB is activated when cells are grown on gamma-resorcylate | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.220 | metabolism | the enzyme gamma-resorcylate catabolic-pathway | Rhodococcus jostii |
4.1.1.103 | physiological function | expression of the tsdA gene confers gamma-resorcylate decarboxylase activity on Escherichia coli. Transcription of the tsdBADC and tsdTX operons required for gamma-resorcylate catabolism is induced during growth on gamma-resorcylate. Inactivation of tsdR derepresses transcription of the tsdBADC and tsdTX operons in the absence of gamma-resorcylate | Rhodococcus jostii |