BRENDA - Enzyme Database

Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols

Wu, X.; Zhang, C.; Orita, I.; Imanaka, T.; Fukui, T.; Xing, X.H.; Appl. Environ. Microbiol. 79, 2209-2217 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.1.1.1
expression in Escherichia coli
Thermococcus kodakarensis
1.1.1.71
expressed in Escherichia coli Rosetta(DE3) cells
Thermococcus kodakarensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.71
Ag+
-
Thermococcus kodakarensis
1.1.1.71
Ca2+
-
Thermococcus kodakarensis
1.1.1.71
Co2+
-
Thermococcus kodakarensis
1.1.1.71
Cu2+
-
Thermococcus kodakarensis
1.1.1.71
Mg2+
slight inhibition
Thermococcus kodakarensis
1.1.1.71
additional information
dithiothreitol and EDTA have no effect on either oxidative or reductive activity
Thermococcus kodakarensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.066
-
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.089
-
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.105
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.113
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.113
-
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.127
-
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
30.2
-
acetoin
pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
61.3
-
2,3-Butanediol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
244
-
1-phenylethanol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.71
0.066
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.089
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.105
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.113
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.113
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.127
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
30.2
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
61.3
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
244
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
27000
-
gel filtration
Thermococcus kodakarensis
1.1.1.1
31000
-
1 * 31000, SDS-PAGE, 1 * 31464, calculated
Thermococcus kodakarensis
1.1.1.1
31464
-
1 * 31000, SDS-PAGE, 1 * 31464, calculated
Thermococcus kodakarensis
1.1.1.71
27000
-
gel filtration
Thermococcus kodakarensis
1.1.1.71
31000
-
1 * 31000, SDS-PAGE
Thermococcus kodakarensis
1.1.1.71
31464
-
-
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.71
(RS)-1-phenylethanol + NAD+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADH + H+
-
-
?
1.1.1.71
(RS)-1-phenylethanol + NADP+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADPH + H+
-
-
?
1.1.1.71
1-(2-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
11.3% activity compared to (RS)-1-phenylethanol
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(2-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
16.4% activity compared to (RS)-1-phenylethanol
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(3-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
315% activity compared to (RS)-1-phenylethanol
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(3-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
205% activity compared to (RS)-1-phenylethanol
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
167% activity compared to (RS)-1-phenylethanol
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-chlorophenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
151% activity compared to (RS)-1-phenylethanol
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-fluorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
119% activity compared to (RS)-1-phenylethanol
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-methylphenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
189% activity compared to (RS)-1-phenylethanol
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
?
1.1.1.71
1-butanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
butanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-heptanol + NAD(P)+
Thermococcus kodakarensis
3.3% activity compared to (RS)-1-phenylethanol
heptanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-hexanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
hexanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-octanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
octanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-pentanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
pentanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-phenyl-1,2-ethanediol + NAD(P)+
Thermococcus kodakarensis
3.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
1-phenyl-1-propanol + NAD(P)+
Thermococcus kodakarensis
13.1% activity compared to (RS)-1-phenylethanol
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
1-phenyl-2-propanol + NAD(P)+
Thermococcus kodakarensis
39.1% activity compared to (RS)-1-phenylethanol
1-phenylpropan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
1-propanol + NAD(P)+
Thermococcus kodakarensis
1.2% activity compared to (RS)-1-phenylethanol
propionaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
2,2,2-trifluoroacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
180% activity compared to acetoin
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
?
1.1.1.71
2-bromoacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
8.7% activity compared to acetoin
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
?
1.1.1.71
2-butanol + NAD(P)+
Thermococcus kodakarensis
22.3% activity compared to (RS)-1-phenylethanol
butan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-chloro-1-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-heptanol + NAD(P)+
Thermococcus kodakarensis
61.6% activity compared to (RS)-1-phenylethanol
heptan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-hexanol + NAD(P)+
Thermococcus kodakarensis
38.7% activity compared to (RS)-1-phenylethanol
hexan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-hexanone + NAD(P)H + H+
Thermococcus kodakarensis
3.6% activity compared to acetoin
hexan-2-ol + NAD(P)+
-
-
?
1.1.1.71
2-octanol + NAD(P)+
Thermococcus kodakarensis
45.8% activity compared to (RS)-1-phenylethanol
octan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-pentanol + NAD(P)+
Thermococcus kodakarensis
30.2% activity compared to (RS)-1-phenylethanol
pentan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
phenylacetaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
2-propanol + NAD(P)+
Thermococcus kodakarensis
5.3% activity compared to (RS)-1-phenylethanol
propan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
acetoin + NAD(P)+
Thermococcus kodakarensis
4.5% activity compared to (RS)-1-phenylethanol
2,3-butanediol + NAD(P)H + H+
-
-
?
1.1.1.71
acetoin + NAD(P)H + H+
Thermococcus kodakarensis
100% activity
butan-2,3-dione + NAD(P)H + H+
-
-
?
1.1.1.71
acetone + NAD(P)H + H+
Thermococcus kodakarensis
4.1% activity compared to acetoin
propan-2-ol + NAD(P)+
-
-
?
1.1.1.71
cyclohexanol + NAD(P)+
Thermococcus kodakarensis
52.3% activity compared to (RS)-1-phenylethanol
cyclohexanone + NAD(P)H + H+
-
-
?
1.1.1.71
cyclohexanone + NAD(P)H + H+
Thermococcus kodakarensis
19.1% activity compared to acetoin
cyclohexanol + NAD(P)+
-
-
?
1.1.1.71
D-arabinose + NAD(P)+
Thermococcus kodakarensis
21.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
dihydroxyacetone + NAD(P)H + H+
Thermococcus kodakarensis
34.8% activity compared to acetoin
?
-
-
?
1.1.1.71
ethanol + NAD(P)+
Thermococcus kodakarensis
0.5% activity compared to (RS)-1-phenylethanol
acetaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
ethyl pyruvate + NAD(P)H + H+
Thermococcus kodakarensis
294% activity compared to acetoin
?
-
-
?
1.1.1.71
hexanal + NAD(P)H + H+
Thermococcus kodakarensis
12.9% activity compared to acetoin
hexan-1-ol + NAD(P)+
-
-
?
1.1.1.71
L-arabinose + NAD(P)+
Thermococcus kodakarensis
5.2% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
meso-2,3-butanediol + NAD+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
meso-2,3-butanediol + NADP+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
methanol + NAD(P)+
Thermococcus kodakarensis
0.2% activity compared to (RS)-1-phenylethanol
formaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
methyl benzoylformate + NAD(P)H + H+
Thermococcus kodakarensis
67% activity compared to acetoin
?
-
-
?
1.1.1.71
additional information
Thermococcus kodakarensis
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
?
-
-
-
1.1.1.71
phenylacetaldehyde + NAD(P)H + H+
Thermococcus kodakarensis
18.6% activity compared to acetoin
2-phenylethan-1-ol + NAD(P)+
-
-
?
1.1.1.71
pyruvic aldehyde + NAD(P)H + H+
Thermococcus kodakarensis
328% activity compared to acetoin
?
-
-
?
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
1.1.1.1
2-propanol
20%, about 110% of initial activity
Thermococcus kodakarensis
1.1.1.1
hexane
50%, about 95% of initial activity
Thermococcus kodakarensis
1.1.1.1
additional information
enzyme shows high resistance to organic solvents
Thermococcus kodakarensis
1.1.1.1
octane
50%, about 90% of initial activity
Thermococcus kodakarensis
1.1.1.71
2-propanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
Acetone
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
dimethylfluoride
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
DMSO
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
Ethanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
ethylacetate
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
1.1.1.71
hexane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
1.1.1.71
Methanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
octane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
1.1.1.71
octanol
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.1
Thermococcus kodakarensis
Q5JI09
-
-
1.1.1.71
Thermococcus kodakarensis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.71
Q sepharose column chromatography
Thermococcus kodakarensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.1
1-(3-bromophenyl)ethanol + NAD+
315% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-bromophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(3-chlorophenyl)ethanol + NAD+
205% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-chlorophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-bromophenyl)ethanol + NAD+
167% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-bromophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-chlorophenyl)ethanol + NAD+
151% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-chlorophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-fluorophenyl)ethanol + NAD+
119% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-fluorophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-methylphenyl)ethanol + NAD+
189% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-methylphenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-phenyl-2-propanol + NAD+
39% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-phenyl-2-propanone + NADH + H+
-
-
-
?
1.1.1.1
1-phenylethanol + NAD+
-
735486
Thermococcus kodakarensis
1-phenylethanone + NADH + H+
-
-
-
?
1.1.1.1
1-phenylethanol + NADP+
-
735486
Thermococcus kodakarensis
1-phenylethanone + NADPH + H+
-
-
-
?
1.1.1.1
2,2,2-trifluoroacetophenone + NADPH + H+
180% of the activity with acetoin
735486
Thermococcus kodakarensis
(R)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
-
-
r
1.1.1.1
2,3-butanediol + NAD+
272% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
acetoin + NADH + H+
-
-
-
r
1.1.1.1
2-heptanol + NAD+
62% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
heptan-2-one + NADH + H+
-
-
-
?
1.1.1.1
2-oxopropanal + NADH + H+
328% of the activity with acetoin
735486
Thermococcus kodakarensis
? + NAD+
-
-
-
r
1.1.1.1
acetoin + NADH + H+
-
735486
Thermococcus kodakarensis
2,3-butanediol + NAD+
-
-
-
r
1.1.1.1
cyclohexanol + NAD+
52% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
cyclohexanone + NADH + H+
-
-
-
?
1.1.1.1
ethyl 2-oxopropanoate + NADPH + H+
294& of the activity with acetoin
735486
Thermococcus kodakarensis
(R)-ethyl 2-hydroxypropanoate + NADP+
-
-
-
r
1.1.1.1
additional information
enzyme reduces aldehydes to (R)-alcohols with more than 99.8% enantiomeric excess
735486
Thermococcus kodakarensis
?
-
-
-
-
1.1.1.71
(RS)-1-phenylethanol + NAD+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADH + H+
-
-
-
?
1.1.1.71
(RS)-1-phenylethanol + NADP+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADPH + H+
-
-
-
?
1.1.1.71
1-(2-bromophenyl)ethanol + NAD(P)+
11.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(2-chlorophenyl)ethanol + NAD(P)+
16.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(3-bromophenyl)ethanol + NAD(P)+
315% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(3-chlorophenyl)ethanol + NAD(P)+
205% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-bromophenyl)ethanol + NAD(P)+
167% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-chlorophenyl)ethanone + NAD(P)+
151% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-fluorophenyl)ethanol + NAD(P)+
119% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-methylphenyl)ethanone + NAD(P)+
189% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-butanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-heptanol + NAD(P)+
3.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-hexanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-octanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-pentanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-phenyl-1,2-ethanediol + NAD(P)+
3.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
1-phenyl-1-propanol + NAD(P)+
13.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-phenyl-2-propanol + NAD(P)+
39.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-propanol + NAD(P)+
1.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propionaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
2,2,2-trifluoroacetophenone + NAD(P)H + H+
180% activity compared to acetoin
735486
Thermococcus kodakarensis
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
-
?
1.1.1.71
2-bromoacetophenone + NAD(P)H + H+
8.7% activity compared to acetoin
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
-
?
1.1.1.71
2-butanol + NAD(P)+
22.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-chloro-1-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-heptanol + NAD(P)+
61.6% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-hexanol + NAD(P)+
38.7% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-hexanone + NAD(P)H + H+
3.6% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-2-ol + NAD(P)+
-
-
-
?
1.1.1.71
2-octanol + NAD(P)+
45.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-pentanol + NAD(P)+
30.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-propanol + NAD(P)+
5.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
acetoin + NAD(P)+
4.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2,3-butanediol + NAD(P)H + H+
-
-
-
?
1.1.1.71
acetoin + NAD(P)H + H+
100% activity
735486
Thermococcus kodakarensis
butan-2,3-dione + NAD(P)H + H+
-
-
-
?
1.1.1.71
acetone + NAD(P)H + H+
4.1% activity compared to acetoin
735486
Thermococcus kodakarensis
propan-2-ol + NAD(P)+
-
-
-
?
1.1.1.71
cyclohexanol + NAD(P)+
52.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
cyclohexanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
cyclohexanone + NAD(P)H + H+
19.1% activity compared to acetoin
735486
Thermococcus kodakarensis
cyclohexanol + NAD(P)+
-
-
-
?
1.1.1.71
D-arabinose + NAD(P)+
21.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
dihydroxyacetone + NAD(P)H + H+
34.8% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
ethanol + NAD(P)+
0.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
acetaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
ethyl pyruvate + NAD(P)H + H+
294% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
hexanal + NAD(P)H + H+
12.9% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-1-ol + NAD(P)+
-
-
-
?
1.1.1.71
L-arabinose + NAD(P)+
5.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
meso-2,3-butanediol + NAD+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
meso-2,3-butanediol + NADP+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
methanol + NAD(P)+
0.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
formaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
methyl benzoylformate + NAD(P)H + H+
67% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
additional information
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
735486
Thermococcus kodakarensis
?
-
-
-
-
1.1.1.71
phenylacetaldehyde + NAD(P)H + H+
18.6% activity compared to acetoin
735486
Thermococcus kodakarensis
2-phenylethan-1-ol + NAD(P)+
-
-
-
?
1.1.1.71
pyruvic aldehyde + NAD(P)H + H+
328% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
monomer
1 * 31000, SDS-PAGE, 1 * 31464, calculated
Thermococcus kodakarensis
1.1.1.71
monomer
1 * 31000, SDS-PAGE; 1 * 31464, calculated from amino acid sequence
Thermococcus kodakarensis
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.1.1
TK0845
-
Thermococcus kodakarensis
1.1.1.71
ADH
-
Thermococcus kodakarensis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.1
90
-
-
Thermococcus kodakarensis
1.1.1.71
90
-
-
Thermococcus kodakarensis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.1
95
-
half-life 4.5 h
Thermococcus kodakarensis
1.1.1.71
95
-
the highly thermostable enzyme has a half-life of 4.5 h at 95C and 16.2 h at 85C
Thermococcus kodakarensis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
10.4
-
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
10.5
-
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
13.7
-
acetoin
pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
15.6
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
17.3
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
30
-
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
30.8
-
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
35.7
-
1-phenylethanol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
43.8
-
2,3-Butanediol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.71
10.4
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
10.5
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
13.7
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
15.6
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
17.3
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
30
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
30.8
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
35.7
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
43.8
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
3
-
reduction of aldehydes
Thermococcus kodakarensis
1.1.1.1
9
-
oxidation reaction of alcohols
Thermococcus kodakarensis
1.1.1.71
3
-
reduction reaction
Thermococcus kodakarensis
1.1.1.71
9
-
oxidation reaction
Thermococcus kodakarensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
6
-
50% of maximum activity for reduction of aldehydes
Thermococcus kodakarensis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Thermococcus kodakarensis
1.1.1.1
NADP+
-
Thermococcus kodakarensis
1.1.1.71
NAD+
-
Thermococcus kodakarensis
1.1.1.71
NADP+
-
Thermococcus kodakarensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.1
expression in Escherichia coli
Thermococcus kodakarensis
1.1.1.71
expressed in Escherichia coli Rosetta(DE3) cells
Thermococcus kodakarensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Thermococcus kodakarensis
1.1.1.1
NADP+
-
Thermococcus kodakarensis
1.1.1.71
NAD+
-
Thermococcus kodakarensis
1.1.1.71
NADP+
-
Thermococcus kodakarensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.71
Ag+
-
Thermococcus kodakarensis
1.1.1.71
Ca2+
-
Thermococcus kodakarensis
1.1.1.71
Co2+
-
Thermococcus kodakarensis
1.1.1.71
Cu2+
-
Thermococcus kodakarensis
1.1.1.71
Mg2+
slight inhibition
Thermococcus kodakarensis
1.1.1.71
additional information
dithiothreitol and EDTA have no effect on either oxidative or reductive activity
Thermococcus kodakarensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.066
-
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.089
-
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.105
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.113
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.113
-
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.127
-
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
30.2
-
acetoin
pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
61.3
-
2,3-Butanediol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
244
-
1-phenylethanol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.71
0.066
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.089
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.105
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.113
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.113
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.127
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
30.2
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
61.3
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
244
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
27000
-
gel filtration
Thermococcus kodakarensis
1.1.1.1
31000
-
1 * 31000, SDS-PAGE, 1 * 31464, calculated
Thermococcus kodakarensis
1.1.1.1
31464
-
1 * 31000, SDS-PAGE, 1 * 31464, calculated
Thermococcus kodakarensis
1.1.1.71
27000
-
gel filtration
Thermococcus kodakarensis
1.1.1.71
31000
-
1 * 31000, SDS-PAGE
Thermococcus kodakarensis
1.1.1.71
31464
-
-
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.71
(RS)-1-phenylethanol + NAD+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADH + H+
-
-
?
1.1.1.71
(RS)-1-phenylethanol + NADP+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADPH + H+
-
-
?
1.1.1.71
1-(2-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
11.3% activity compared to (RS)-1-phenylethanol
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(2-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
16.4% activity compared to (RS)-1-phenylethanol
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(3-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
315% activity compared to (RS)-1-phenylethanol
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(3-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
205% activity compared to (RS)-1-phenylethanol
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
167% activity compared to (RS)-1-phenylethanol
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-chlorophenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
151% activity compared to (RS)-1-phenylethanol
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-fluorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
119% activity compared to (RS)-1-phenylethanol
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
?
1.1.1.71
1-(4-methylphenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
189% activity compared to (RS)-1-phenylethanol
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
?
1.1.1.71
1-butanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
butanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-heptanol + NAD(P)+
Thermococcus kodakarensis
3.3% activity compared to (RS)-1-phenylethanol
heptanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-hexanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
hexanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-octanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
octanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-pentanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
pentanal + NAD(P)H + H+
-
-
?
1.1.1.71
1-phenyl-1,2-ethanediol + NAD(P)+
Thermococcus kodakarensis
3.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
1-phenyl-1-propanol + NAD(P)+
Thermococcus kodakarensis
13.1% activity compared to (RS)-1-phenylethanol
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
1-phenyl-2-propanol + NAD(P)+
Thermococcus kodakarensis
39.1% activity compared to (RS)-1-phenylethanol
1-phenylpropan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
1-propanol + NAD(P)+
Thermococcus kodakarensis
1.2% activity compared to (RS)-1-phenylethanol
propionaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
2,2,2-trifluoroacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
180% activity compared to acetoin
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
?
1.1.1.71
2-bromoacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
8.7% activity compared to acetoin
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
?
1.1.1.71
2-butanol + NAD(P)+
Thermococcus kodakarensis
22.3% activity compared to (RS)-1-phenylethanol
butan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-chloro-1-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-heptanol + NAD(P)+
Thermococcus kodakarensis
61.6% activity compared to (RS)-1-phenylethanol
heptan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-hexanol + NAD(P)+
Thermococcus kodakarensis
38.7% activity compared to (RS)-1-phenylethanol
hexan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-hexanone + NAD(P)H + H+
Thermococcus kodakarensis
3.6% activity compared to acetoin
hexan-2-ol + NAD(P)+
-
-
?
1.1.1.71
2-octanol + NAD(P)+
Thermococcus kodakarensis
45.8% activity compared to (RS)-1-phenylethanol
octan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-pentanol + NAD(P)+
Thermococcus kodakarensis
30.2% activity compared to (RS)-1-phenylethanol
pentan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
2-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
phenylacetaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
2-propanol + NAD(P)+
Thermococcus kodakarensis
5.3% activity compared to (RS)-1-phenylethanol
propan-2-one + NAD(P)H + H+
-
-
?
1.1.1.71
acetoin + NAD(P)+
Thermococcus kodakarensis
4.5% activity compared to (RS)-1-phenylethanol
2,3-butanediol + NAD(P)H + H+
-
-
?
1.1.1.71
acetoin + NAD(P)H + H+
Thermococcus kodakarensis
100% activity
butan-2,3-dione + NAD(P)H + H+
-
-
?
1.1.1.71
acetone + NAD(P)H + H+
Thermococcus kodakarensis
4.1% activity compared to acetoin
propan-2-ol + NAD(P)+
-
-
?
1.1.1.71
cyclohexanol + NAD(P)+
Thermococcus kodakarensis
52.3% activity compared to (RS)-1-phenylethanol
cyclohexanone + NAD(P)H + H+
-
-
?
1.1.1.71
cyclohexanone + NAD(P)H + H+
Thermococcus kodakarensis
19.1% activity compared to acetoin
cyclohexanol + NAD(P)+
-
-
?
1.1.1.71
D-arabinose + NAD(P)+
Thermococcus kodakarensis
21.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
dihydroxyacetone + NAD(P)H + H+
Thermococcus kodakarensis
34.8% activity compared to acetoin
?
-
-
?
1.1.1.71
ethanol + NAD(P)+
Thermococcus kodakarensis
0.5% activity compared to (RS)-1-phenylethanol
acetaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
ethyl pyruvate + NAD(P)H + H+
Thermococcus kodakarensis
294% activity compared to acetoin
?
-
-
?
1.1.1.71
hexanal + NAD(P)H + H+
Thermococcus kodakarensis
12.9% activity compared to acetoin
hexan-1-ol + NAD(P)+
-
-
?
1.1.1.71
L-arabinose + NAD(P)+
Thermococcus kodakarensis
5.2% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
meso-2,3-butanediol + NAD+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
meso-2,3-butanediol + NADP+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
1.1.1.71
methanol + NAD(P)+
Thermococcus kodakarensis
0.2% activity compared to (RS)-1-phenylethanol
formaldehyde + NAD(P)H + H+
-
-
?
1.1.1.71
methyl benzoylformate + NAD(P)H + H+
Thermococcus kodakarensis
67% activity compared to acetoin
?
-
-
?
1.1.1.71
additional information
Thermococcus kodakarensis
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
?
-
-
-
1.1.1.71
phenylacetaldehyde + NAD(P)H + H+
Thermococcus kodakarensis
18.6% activity compared to acetoin
2-phenylethan-1-ol + NAD(P)+
-
-
?
1.1.1.71
pyruvic aldehyde + NAD(P)H + H+
Thermococcus kodakarensis
328% activity compared to acetoin
?
-
-
?
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
1.1.1.1
2-propanol
20%, about 110% of initial activity
Thermococcus kodakarensis
1.1.1.1
hexane
50%, about 95% of initial activity
Thermococcus kodakarensis
1.1.1.1
additional information
enzyme shows high resistance to organic solvents
Thermococcus kodakarensis
1.1.1.1
octane
50%, about 90% of initial activity
Thermococcus kodakarensis
1.1.1.71
2-propanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
Acetone
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
dimethylfluoride
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
DMSO
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
Ethanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
ethylacetate
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
1.1.1.71
hexane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
1.1.1.71
Methanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
1.1.1.71
octane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
1.1.1.71
octanol
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.71
Q sepharose column chromatography
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.1
1-(3-bromophenyl)ethanol + NAD+
315% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-bromophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(3-chlorophenyl)ethanol + NAD+
205% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-chlorophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-bromophenyl)ethanol + NAD+
167% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-bromophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-chlorophenyl)ethanol + NAD+
151% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-chlorophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-fluorophenyl)ethanol + NAD+
119% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-fluorophenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-(4-methylphenyl)ethanol + NAD+
189% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-methylphenyl)ethanone + NADH + H+
-
-
-
?
1.1.1.1
1-phenyl-2-propanol + NAD+
39% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
1-phenyl-2-propanone + NADH + H+
-
-
-
?
1.1.1.1
1-phenylethanol + NAD+
-
735486
Thermococcus kodakarensis
1-phenylethanone + NADH + H+
-
-
-
?
1.1.1.1
1-phenylethanol + NADP+
-
735486
Thermococcus kodakarensis
1-phenylethanone + NADPH + H+
-
-
-
?
1.1.1.1
2,2,2-trifluoroacetophenone + NADPH + H+
180% of the activity with acetoin
735486
Thermococcus kodakarensis
(R)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
-
-
r
1.1.1.1
2,3-butanediol + NAD+
272% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
acetoin + NADH + H+
-
-
-
r
1.1.1.1
2-heptanol + NAD+
62% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
heptan-2-one + NADH + H+
-
-
-
?
1.1.1.1
2-oxopropanal + NADH + H+
328% of the activity with acetoin
735486
Thermococcus kodakarensis
? + NAD+
-
-
-
r
1.1.1.1
acetoin + NADH + H+
-
735486
Thermococcus kodakarensis
2,3-butanediol + NAD+
-
-
-
r
1.1.1.1
cyclohexanol + NAD+
52% of the activity with 1-phenylethanol
735486
Thermococcus kodakarensis
cyclohexanone + NADH + H+
-
-
-
?
1.1.1.1
ethyl 2-oxopropanoate + NADPH + H+
294& of the activity with acetoin
735486
Thermococcus kodakarensis
(R)-ethyl 2-hydroxypropanoate + NADP+
-
-
-
r
1.1.1.1
additional information
enzyme reduces aldehydes to (R)-alcohols with more than 99.8% enantiomeric excess
735486
Thermococcus kodakarensis
?
-
-
-
-
1.1.1.71
(RS)-1-phenylethanol + NAD+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADH + H+
-
-
-
?
1.1.1.71
(RS)-1-phenylethanol + NADP+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADPH + H+
-
-
-
?
1.1.1.71
1-(2-bromophenyl)ethanol + NAD(P)+
11.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(2-chlorophenyl)ethanol + NAD(P)+
16.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(3-bromophenyl)ethanol + NAD(P)+
315% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(3-chlorophenyl)ethanol + NAD(P)+
205% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-bromophenyl)ethanol + NAD(P)+
167% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-chlorophenyl)ethanone + NAD(P)+
151% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-fluorophenyl)ethanol + NAD(P)+
119% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-(4-methylphenyl)ethanone + NAD(P)+
189% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-butanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-heptanol + NAD(P)+
3.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-hexanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-octanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-pentanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentanal + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-phenyl-1,2-ethanediol + NAD(P)+
3.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
1-phenyl-1-propanol + NAD(P)+
13.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-phenyl-2-propanol + NAD(P)+
39.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
1-propanol + NAD(P)+
1.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propionaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
2,2,2-trifluoroacetophenone + NAD(P)H + H+
180% activity compared to acetoin
735486
Thermococcus kodakarensis
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
-
?
1.1.1.71
2-bromoacetophenone + NAD(P)H + H+
8.7% activity compared to acetoin
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
-
?
1.1.1.71
2-butanol + NAD(P)+
22.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-chloro-1-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-heptanol + NAD(P)+
61.6% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-hexanol + NAD(P)+
38.7% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-hexanone + NAD(P)H + H+
3.6% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-2-ol + NAD(P)+
-
-
-
?
1.1.1.71
2-octanol + NAD(P)+
45.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-pentanol + NAD(P)+
30.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
2-propanol + NAD(P)+
5.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propan-2-one + NAD(P)H + H+
-
-
-
?
1.1.1.71
acetoin + NAD(P)+
4.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2,3-butanediol + NAD(P)H + H+
-
-
-
?
1.1.1.71
acetoin + NAD(P)H + H+
100% activity
735486
Thermococcus kodakarensis
butan-2,3-dione + NAD(P)H + H+
-
-
-
?
1.1.1.71
acetone + NAD(P)H + H+
4.1% activity compared to acetoin
735486
Thermococcus kodakarensis
propan-2-ol + NAD(P)+
-
-
-
?
1.1.1.71
cyclohexanol + NAD(P)+
52.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
cyclohexanone + NAD(P)H + H+
-
-
-
?
1.1.1.71
cyclohexanone + NAD(P)H + H+
19.1% activity compared to acetoin
735486
Thermococcus kodakarensis
cyclohexanol + NAD(P)+
-
-
-
?
1.1.1.71
D-arabinose + NAD(P)+
21.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
dihydroxyacetone + NAD(P)H + H+
34.8% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
ethanol + NAD(P)+
0.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
acetaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
ethyl pyruvate + NAD(P)H + H+
294% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
hexanal + NAD(P)H + H+
12.9% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-1-ol + NAD(P)+
-
-
-
?
1.1.1.71
L-arabinose + NAD(P)+
5.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
meso-2,3-butanediol + NAD+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
meso-2,3-butanediol + NADP+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
methanol + NAD(P)+
0.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
formaldehyde + NAD(P)H + H+
-
-
-
?
1.1.1.71
methyl benzoylformate + NAD(P)H + H+
67% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
1.1.1.71
additional information
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
735486
Thermococcus kodakarensis
?
-
-
-
-
1.1.1.71
phenylacetaldehyde + NAD(P)H + H+
18.6% activity compared to acetoin
735486
Thermococcus kodakarensis
2-phenylethan-1-ol + NAD(P)+
-
-
-
?
1.1.1.71
pyruvic aldehyde + NAD(P)H + H+
328% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
monomer
1 * 31000, SDS-PAGE, 1 * 31464, calculated
Thermococcus kodakarensis
1.1.1.71
monomer
1 * 31000, SDS-PAGE; 1 * 31464, calculated from amino acid sequence
Thermococcus kodakarensis
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.1
90
-
-
Thermococcus kodakarensis
1.1.1.71
90
-
-
Thermococcus kodakarensis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.1
95
-
half-life 4.5 h
Thermococcus kodakarensis
1.1.1.71
95
-
the highly thermostable enzyme has a half-life of 4.5 h at 95C and 16.2 h at 85C
Thermococcus kodakarensis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
10.4
-
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
10.5
-
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
13.7
-
acetoin
pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
15.6
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
17.3
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
30
-
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
30.8
-
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
35.7
-
1-phenylethanol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
43.8
-
2,3-Butanediol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.71
10.4
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
10.5
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
13.7
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
15.6
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
17.3
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
30
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
30.8
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
35.7
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
43.8
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
3
-
reduction of aldehydes
Thermococcus kodakarensis
1.1.1.1
9
-
oxidation reaction of alcohols
Thermococcus kodakarensis
1.1.1.71
3
-
reduction reaction
Thermococcus kodakarensis
1.1.1.71
9
-
oxidation reaction
Thermococcus kodakarensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
6
-
50% of maximum activity for reduction of aldehydes
Thermococcus kodakarensis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.15
-
1-phenylethanol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.45
-
acetoin
pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.71
-
2,3-Butanediol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
117
-
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
149
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
153
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
159
-
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
243
-
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
265
-
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.71
0.15
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.45
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.71
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
117
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
149
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
153
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
159
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
243
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
265
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.15
-
1-phenylethanol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.45
-
acetoin
pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
0.71
-
2,3-Butanediol
pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
117
-
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
149
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
153
-
NADH
cosubstrate acetoin, pH 6.0, 70C
Thermococcus kodakarensis
1.1.1.1
159
-
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
243
-
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.1
265
-
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70C
Thermococcus kodakarensis
1.1.1.71
0.15
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.45
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
0.71
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
117
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
149
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
153
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
159
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
243
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis
1.1.1.71
265
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70C
Thermococcus kodakarensis