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Literature summary extracted from

  • Wu, X.; Zhang, C.; Orita, I.; Imanaka, T.; Fukui, T.; Xing, X.H.
    Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols (2013), Appl. Environ. Microbiol., 79, 2209-2217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Thermococcus kodakarensis
1.1.1.71 expressed in Escherichia coli Rosetta(DE3) cells Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.71 Ag+
-
 Thermococcus kodakarensis
1.1.1.71 Ca2+
-
 Thermococcus kodakarensis
1.1.1.71 Co2+
-
 Thermococcus kodakarensis
1.1.1.71 Cu2+
-
 Thermococcus kodakarensis
1.1.1.71 Mg2+ slight inhibition Thermococcus kodakarensis
1.1.1.71 additional information dithiothreitol and EDTA have no effect on either oxidative or reductive activity Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.066
-
 NADP+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 0.089
-
 NAD+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 0.105
-
 NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 0.113
-
 NADP+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 0.113
-
 NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 0.127
-
 NAD+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 30.2
-
 acetoin pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 61.3
-
 2,3-Butanediol pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 244
-
 1-phenylethanol pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.71 0.066
-
 NADP+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 0.089
-
 NAD+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 0.105
-
 NADH with acetoin as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 0.113
-
 NADPH with acetoin as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 0.113
-
 NADP+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 0.127
-
 NAD+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 30.2
-
 acetoin with NADH as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 61.3
-
 meso-2,3-butanediol with NAD+ as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 244
-
 (RS)-1-phenylethanol with NAD+ as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 27000
-
 gel filtration Thermococcus kodakarensis
1.1.1.1 31000
-
 1 * 31000, SDS-PAGE, 1 * 31464, calculated Thermococcus kodakarensis
1.1.1.1 31464
-
 1 * 31000, SDS-PAGE, 1 * 31464, calculated Thermococcus kodakarensis
1.1.1.71 27000
-
 gel filtration Thermococcus kodakarensis
1.1.1.71 31000
-
 1 * 31000, SDS-PAGE Thermococcus kodakarensis
1.1.1.71 31464
-
-
 Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.71 (RS)-1-phenylethanol + NAD+ Thermococcus kodakarensis 100% activity 1-phenylethanone + NADH + H+
-
 ?
1.1.1.71 (RS)-1-phenylethanol + NADP+ Thermococcus kodakarensis 100% activity 1-phenylethanone + NADPH + H+
-
 ?
1.1.1.71 1-(2-bromophenyl)ethanol + NAD(P)+ Thermococcus kodakarensis 11.3% activity compared to (RS)-1-phenylethanol 1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(2-chlorophenyl)ethanol + NAD(P)+ Thermococcus kodakarensis 16.4% activity compared to (RS)-1-phenylethanol 1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(3-bromophenyl)ethanol + NAD(P)+ Thermococcus kodakarensis 315% activity compared to (RS)-1-phenylethanol 1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(3-chlorophenyl)ethanol + NAD(P)+ Thermococcus kodakarensis 205% activity compared to (RS)-1-phenylethanol 1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-bromophenyl)ethanol + NAD(P)+ Thermococcus kodakarensis 167% activity compared to (RS)-1-phenylethanol 1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-chlorophenyl)ethanone + NAD(P)+ Thermococcus kodakarensis 151% activity compared to (RS)-1-phenylethanol 1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-fluorophenyl)ethanol + NAD(P)+ Thermococcus kodakarensis 119% activity compared to (RS)-1-phenylethanol 1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-methylphenyl)ethanone + NAD(P)+ Thermococcus kodakarensis 189% activity compared to (RS)-1-phenylethanol 1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
 ?
1.1.1.71 1-butanol + NAD(P)+ Thermococcus kodakarensis 2.3% activity compared to (RS)-1-phenylethanol butanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-heptanol + NAD(P)+ Thermococcus kodakarensis 3.3% activity compared to (RS)-1-phenylethanol heptanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-hexanol + NAD(P)+ Thermococcus kodakarensis 2.8% activity compared to (RS)-1-phenylethanol hexanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-octanol + NAD(P)+ Thermococcus kodakarensis 2.3% activity compared to (RS)-1-phenylethanol octanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-pentanol + NAD(P)+ Thermococcus kodakarensis 2.8% activity compared to (RS)-1-phenylethanol pentanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-phenyl-1,2-ethanediol + NAD(P)+ Thermococcus kodakarensis 3.4% activity compared to (RS)-1-phenylethanol ?
-
 ?
1.1.1.71 1-phenyl-1-propanol + NAD(P)+ Thermococcus kodakarensis 13.1% activity compared to (RS)-1-phenylethanol 1-phenylpropionaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 1-phenyl-2-propanol + NAD(P)+ Thermococcus kodakarensis 39.1% activity compared to (RS)-1-phenylethanol 1-phenylpropan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 1-propanol + NAD(P)+ Thermococcus kodakarensis 1.2% activity compared to (RS)-1-phenylethanol propionaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 2,2,2-trifluoroacetophenone + NAD(P)H + H+ Thermococcus kodakarensis 180% activity compared to acetoin 2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
 ?
1.1.1.71 2-bromoacetophenone + NAD(P)H + H+ Thermococcus kodakarensis 8.7% activity compared to acetoin 1-(2-bromophenyl)ethanone + NAD(P)+
-
 ?
1.1.1.71 2-butanol + NAD(P)+ Thermococcus kodakarensis 22.3% activity compared to (RS)-1-phenylethanol butan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-chloro-1-phenylethanol + NAD(P)+ Thermococcus kodakarensis 0.3% activity compared to (RS)-1-phenylethanol 2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-heptanol + NAD(P)+ Thermococcus kodakarensis 61.6% activity compared to (RS)-1-phenylethanol heptan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-hexanol + NAD(P)+ Thermococcus kodakarensis 38.7% activity compared to (RS)-1-phenylethanol hexan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-hexanone + NAD(P)H + H+ Thermococcus kodakarensis 3.6% activity compared to acetoin hexan-2-ol + NAD(P)+
-
 ?
1.1.1.71 2-octanol + NAD(P)+ Thermococcus kodakarensis 45.8% activity compared to (RS)-1-phenylethanol octan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-pentanol + NAD(P)+ Thermococcus kodakarensis 30.2% activity compared to (RS)-1-phenylethanol pentan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-phenylethanol + NAD(P)+ Thermococcus kodakarensis 0.3% activity compared to (RS)-1-phenylethanol phenylacetaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 2-propanol + NAD(P)+ Thermococcus kodakarensis 5.3% activity compared to (RS)-1-phenylethanol propan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 acetoin + NAD(P)+ Thermococcus kodakarensis 100% activity butan-2,3-dione + NAD(P)H + H+
-
 ?
1.1.1.71 acetoin + NAD(P)H + H+ Thermococcus kodakarensis 4.5% activity compared to (RS)-1-phenylethanol 2,3-butanediol + NAD(P)+
-
 ?
1.1.1.71 acetone + NAD(P)H + H+ Thermococcus kodakarensis 4.1% activity compared to acetoin propan-2-ol + NAD(P)+
-
 ?
1.1.1.71 cyclohexanol + NAD(P)+ Thermococcus kodakarensis 52.3% activity compared to (RS)-1-phenylethanol cyclohexanone + NAD(P)H + H+
-
 ?
1.1.1.71 cyclohexanone + NAD(P)H + H+ Thermococcus kodakarensis 19.1% activity compared to acetoin cyclohexanol + NAD(P)+
-
 ?
1.1.1.71 D-arabinose + NAD(P)+ Thermococcus kodakarensis 21.4% activity compared to (RS)-1-phenylethanol ?
-
 ?
1.1.1.71 dihydroxyacetone + NAD(P)H + H+ Thermococcus kodakarensis 34.8% activity compared to acetoin ?
-
 ?
1.1.1.71 ethanol + NAD(P)+ Thermococcus kodakarensis 0.5% activity compared to (RS)-1-phenylethanol acetaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 ethyl pyruvate + NAD(P)H + H+ Thermococcus kodakarensis 294% activity compared to acetoin ?
-
 ?
1.1.1.71 hexanal + NAD(P)H + H+ Thermococcus kodakarensis 12.9% activity compared to acetoin hexan-1-ol + NAD(P)+
-
 ?
1.1.1.71 L-arabinose + NAD(P)+ Thermococcus kodakarensis 5.2% activity compared to (RS)-1-phenylethanol ?
-
 ?
1.1.1.71 meso-2,3-butanediol + NAD+ Thermococcus kodakarensis 272% activity compared to (RS)-1-phenylethanol ?
-
 ?
1.1.1.71 meso-2,3-butanediol + NADP+ Thermococcus kodakarensis 272% activity compared to (RS)-1-phenylethanol ?
-
 ?
1.1.1.71 methanol + NAD(P)+ Thermococcus kodakarensis 0.2% activity compared to (RS)-1-phenylethanol formaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 methyl benzoylformate + NAD(P)H + H+ Thermococcus kodakarensis 67% activity compared to acetoin ?
-
 ?
1.1.1.71 additional information Thermococcus kodakarensis no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone ?
-
 ?
1.1.1.71 phenylacetaldehyde + NAD(P)H + H+ Thermococcus kodakarensis 18.6% activity compared to acetoin 2-phenylethan-1-ol + NAD(P)+
-
 ?
1.1.1.71 pyruvic aldehyde + NAD(P)H + H+ Thermococcus kodakarensis 328% activity compared to acetoin ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.1 2-propanol 20%, about 110% of initial activity Thermococcus kodakarensis
1.1.1.1 hexane 50%, about 95% of initial activity Thermococcus kodakarensis
1.1.1.1 additional information enzyme shows high resistance to organic solvents Thermococcus kodakarensis
1.1.1.1 octane 50%, about 90% of initial activity Thermococcus kodakarensis
1.1.1.71 2-propanol high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v) Thermococcus kodakarensis
1.1.1.71 Acetone high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v) Thermococcus kodakarensis
1.1.1.71 dimethylfluoride high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v) Thermococcus kodakarensis
1.1.1.71 DMSO high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v) Thermococcus kodakarensis
1.1.1.71 Ethanol high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v) Thermococcus kodakarensis
1.1.1.71 ethylacetate high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v) Thermococcus kodakarensis
1.1.1.71 hexane high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v) Thermococcus kodakarensis
1.1.1.71 Methanol high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v) Thermococcus kodakarensis
1.1.1.71 octane high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v) Thermococcus kodakarensis
1.1.1.71 octanol high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v) Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Thermococcus kodakarensis Q5JI09
-
-
1.1.1.71 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71 Q sepharose column chromatography Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-(3-bromophenyl)ethanol + NAD+ 315% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(3-bromophenyl)ethanone + NADH + H+
-
 ?
1.1.1.1 1-(3-chlorophenyl)ethanol + NAD+ 205% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(3-chlorophenyl)ethanone + NADH + H+
-
 ?
1.1.1.1 1-(4-bromophenyl)ethanol + NAD+ 167% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-bromophenyl)ethanone + NADH + H+
-
 ?
1.1.1.1 1-(4-chlorophenyl)ethanol + NAD+ 151% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-chlorophenyl)ethanone + NADH + H+
-
 ?
1.1.1.1 1-(4-fluorophenyl)ethanol + NAD+ 119% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-fluorophenyl)ethanone + NADH + H+
-
 ?
1.1.1.1 1-(4-methylphenyl)ethanol + NAD+ 189% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-(4-methylphenyl)ethanone + NADH + H+
-
 ?
1.1.1.1 1-phenyl-2-propanol + NAD+ 39% of the activity with 1-phenylethanol Thermococcus kodakarensis 1-phenyl-2-propanone + NADH + H+
-
 ?
1.1.1.1 1-phenylethanol + NAD+
-
 Thermococcus kodakarensis 1-phenylethanone + NADH + H+
-
 ?
1.1.1.1 1-phenylethanol + NADP+
-
 Thermococcus kodakarensis 1-phenylethanone + NADPH + H+
-
 ?
1.1.1.1 2,2,2-trifluoroacetophenone + NADPH + H+ 180% of the activity with acetoin Thermococcus kodakarensis (R)-2,2,2-trifluoro-1-phenylethanol + NADP+
-
 r
1.1.1.1 2,3-butanediol + NAD+ 272% of the activity with 1-phenylethanol Thermococcus kodakarensis acetoin + NADH + H+
-
 r
1.1.1.1 2-heptanol + NAD+ 62% of the activity with 1-phenylethanol Thermococcus kodakarensis heptan-2-one + NADH + H+
-
 ?
1.1.1.1 2-oxopropanal + NADH + H+ 328% of the activity with acetoin Thermococcus kodakarensis ? + NAD+
-
 r
1.1.1.1 acetoin + NADH + H+
-
 Thermococcus kodakarensis 2,3-butanediol + NAD+
-
 r
1.1.1.1 cyclohexanol + NAD+ 52% of the activity with 1-phenylethanol Thermococcus kodakarensis cyclohexanone + NADH + H+
-
 ?
1.1.1.1 ethyl 2-oxopropanoate + NADPH + H+ 294& of the activity with acetoin Thermococcus kodakarensis (R)-ethyl 2-hydroxypropanoate + NADP+
-
 r
1.1.1.1 additional information enzyme reduces aldehydes to (R)-alcohols with more than 99.8% enantiomeric excess Thermococcus kodakarensis ?
-
 ?
1.1.1.71 (RS)-1-phenylethanol + NAD+ 100% activity Thermococcus kodakarensis 1-phenylethanone + NADH + H+
-
 ?
1.1.1.71 (RS)-1-phenylethanol + NADP+ 100% activity Thermococcus kodakarensis 1-phenylethanone + NADPH + H+
-
 ?
1.1.1.71 1-(2-bromophenyl)ethanol + NAD(P)+ 11.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(2-chlorophenyl)ethanol + NAD(P)+ 16.4% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(3-bromophenyl)ethanol + NAD(P)+ 315% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(3-chlorophenyl)ethanol + NAD(P)+ 205% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-bromophenyl)ethanol + NAD(P)+ 167% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-chlorophenyl)ethanone + NAD(P)+ 151% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-fluorophenyl)ethanol + NAD(P)+ 119% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
 ?
1.1.1.71 1-(4-methylphenyl)ethanone + NAD(P)+ 189% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
 ?
1.1.1.71 1-butanol + NAD(P)+ 2.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis butanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-heptanol + NAD(P)+ 3.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis heptanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-hexanol + NAD(P)+ 2.8% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis hexanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-octanol + NAD(P)+ 2.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis octanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-pentanol + NAD(P)+ 2.8% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis pentanal + NAD(P)H + H+
-
 ?
1.1.1.71 1-phenyl-1,2-ethanediol + NAD(P)+ 3.4% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ?
-
 ?
1.1.1.71 1-phenyl-1-propanol + NAD(P)+ 13.1% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-phenylpropionaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 1-phenyl-2-propanol + NAD(P)+ 39.1% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-phenylpropan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 1-propanol + NAD(P)+ 1.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis propionaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 2,2,2-trifluoroacetophenone + NAD(P)H + H+ 180% activity compared to acetoin Thermococcus kodakarensis 2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
 ?
1.1.1.71 2-bromoacetophenone + NAD(P)H + H+ 8.7% activity compared to acetoin Thermococcus kodakarensis 1-(2-bromophenyl)ethanone + NAD(P)+
-
 ?
1.1.1.71 2-butanol + NAD(P)+ 22.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis butan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-chloro-1-phenylethanol + NAD(P)+ 0.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-heptanol + NAD(P)+ 61.6% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis heptan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-hexanol + NAD(P)+ 38.7% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis hexan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-hexanone + NAD(P)H + H+ 3.6% activity compared to acetoin Thermococcus kodakarensis hexan-2-ol + NAD(P)+
-
 ?
1.1.1.71 2-octanol + NAD(P)+ 45.8% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis octan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-pentanol + NAD(P)+ 30.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis pentan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 2-phenylethanol + NAD(P)+ 0.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis phenylacetaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 2-propanol + NAD(P)+ 5.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis propan-2-one + NAD(P)H + H+
-
 ?
1.1.1.71 acetoin + NAD(P)+ 100% activity Thermococcus kodakarensis butan-2,3-dione + NAD(P)H + H+
-
 ?
1.1.1.71 acetoin + NAD(P)H + H+ 4.5% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 2,3-butanediol + NAD(P)+
-
 ?
1.1.1.71 acetone + NAD(P)H + H+ 4.1% activity compared to acetoin Thermococcus kodakarensis propan-2-ol + NAD(P)+
-
 ?
1.1.1.71 cyclohexanol + NAD(P)+ 52.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis cyclohexanone + NAD(P)H + H+
-
 ?
1.1.1.71 cyclohexanone + NAD(P)H + H+ 19.1% activity compared to acetoin Thermococcus kodakarensis cyclohexanol + NAD(P)+
-
 ?
1.1.1.71 D-arabinose + NAD(P)+ 21.4% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ?
-
 ?
1.1.1.71 dihydroxyacetone + NAD(P)H + H+ 34.8% activity compared to acetoin Thermococcus kodakarensis ?
-
 ?
1.1.1.71 ethanol + NAD(P)+ 0.5% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis acetaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 ethyl pyruvate + NAD(P)H + H+ 294% activity compared to acetoin Thermococcus kodakarensis ?
-
 ?
1.1.1.71 hexanal + NAD(P)H + H+ 12.9% activity compared to acetoin Thermococcus kodakarensis hexan-1-ol + NAD(P)+
-
 ?
1.1.1.71 L-arabinose + NAD(P)+ 5.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ?
-
 ?
1.1.1.71 meso-2,3-butanediol + NAD+ 272% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ?
-
 ?
1.1.1.71 meso-2,3-butanediol + NADP+ 272% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ?
-
 ?
1.1.1.71 methanol + NAD(P)+ 0.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis formaldehyde + NAD(P)H + H+
-
 ?
1.1.1.71 methyl benzoylformate + NAD(P)H + H+ 67% activity compared to acetoin Thermococcus kodakarensis ?
-
 ?
1.1.1.71 additional information no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone Thermococcus kodakarensis ?
-
 ?
1.1.1.71 phenylacetaldehyde + NAD(P)H + H+ 18.6% activity compared to acetoin Thermococcus kodakarensis 2-phenylethan-1-ol + NAD(P)+
-
 ?
1.1.1.71 pyruvic aldehyde + NAD(P)H + H+ 328% activity compared to acetoin Thermococcus kodakarensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 monomer 1 * 31000, SDS-PAGE, 1 * 31464, calculated Thermococcus kodakarensis
1.1.1.71 monomer 1 * 31000, SDS-PAGE Thermococcus kodakarensis
1.1.1.71 monomer 1 * 31464, calculated from amino acid sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 TK0845
-
 Thermococcus kodakarensis
1.1.1.71 ADH
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 90
-
-
 Thermococcus kodakarensis
1.1.1.71 90
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 95
-
 half-life 4.5 h Thermococcus kodakarensis
1.1.1.71 95
-
 the highly thermostable enzyme has a half-life of 4.5 h at 95°C and 16.2 h at 85°C Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 10.4
-
 NAD+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 10.5
-
 NADP+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 13.7
-
 acetoin pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 15.6
-
 NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 17.3
-
 NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 30
-
 NADP+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 30.8
-
 NAD+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 35.7
-
 1-phenylethanol pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 43.8
-
 2,3-Butanediol pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.71 10.4
-
 NAD+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 10.5
-
 NADP+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 13.7
-
 acetoin with NADH as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 15.6
-
 NADH with acetoin as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 17.3
-
 NADPH with acetoin as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 30
-
 NADP+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 30.8
-
 NAD+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 35.7
-
 (RS)-1-phenylethanol with NAD+ as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 43.8
-
 meso-2,3-butanediol with NAD+ as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 3
-
 reduction of aldehydes Thermococcus kodakarensis
1.1.1.1 9
-
 oxidation reaction of alcohols Thermococcus kodakarensis
1.1.1.71 3
-
 reduction reaction Thermococcus kodakarensis
1.1.1.71 9
-
 oxidation reaction Thermococcus kodakarensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 6
-
 50% of maximum activity for reduction of aldehydes Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Thermococcus kodakarensis
1.1.1.1 NADP+
-
 Thermococcus kodakarensis
1.1.1.71 NAD+
-
 Thermococcus kodakarensis
1.1.1.71 NADP+
-
 Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 0.15
-
 1-phenylethanol pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 0.45
-
 acetoin pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 0.71
-
 2,3-Butanediol pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 117
-
 NAD+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 149
-
 NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 153
-
 NADH cosubstrate acetoin, pH 6.0, 70°C Thermococcus kodakarensis
1.1.1.1 159
-
 NADP+ cosubstrate 1-phenylethanol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 243
-
 NAD+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.1 265
-
 NADP+ cosubstrate 2,3-butanediol, pH 9.0, 70°C Thermococcus kodakarensis
1.1.1.71 0.15
-
 (RS)-1-phenylethanol with NAD+ as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 0.45
-
 acetoin with NADH as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 0.71
-
 meso-2,3-butanediol with NAD+ as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 117
-
 NAD+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 149
-
 NADH with acetoin as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 153
-
 NADPH with acetoin as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 159
-
 NADP+ with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 243
-
 NAD+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis
1.1.1.71 265
-
 NADP+ with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C Thermococcus kodakarensis