Any feedback?
Literature summary extracted from
- An acidothermophilic functionally active novel GH12 family endoglucanase from Aspergillus niger HO: purification, characterization and molecular interaction studies (2015), Antonie Van Leeuwenhoek, 107, 103-117.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | degradation | hydrolysis of 2% carboxymethyl cellulose with purified enzyme at its optimum temperature and pH results in complete hydrolysis within 2 h yielding 18% cellotriose, 72% cellobiose and 10% glucose | Aspergillus niger |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | molecular docking studies reveal Glu116 and Glu204 as important for functional interaction with carboxymethylcellulose and show hydrogen bonding with Asp99, Glu116, Glu204 and hydrophobic interactions with Trp22, Val58, Tyr61, Phe101, Met118, Trp120, Pro129, Ile130, Thr160 and Phe206 | Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | Cu2+ | strong inhibition | Aspergillus niger |  |
| 3.2.1.4 | Hg2+ | strong inhibition | Aspergillus niger | |
| 3.2.1.4 | Mn2+ | strong inhibition | Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.4 | additional information | - |
carboxymethyl cellulose | Km-value is 2.5 mg/ml, pH 3.5, 70°C | Aspergillus niger |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | Ca2+ | stimulation | Aspergillus niger | |
| 3.2.1.4 | Co2+ | stimulation | Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 55000 | - |
x * 55000, SDS-PAGE | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Aspergillus niger | O74705 | - |
- |
| 3.2.1.4 | Aspergillus niger HO | O74705 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | - |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 43.5 | - |
pH 3.5, 70°C | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucose | 14% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Aspergillus niger HO | 4-nitrophenol + beta-D-glucose | 14% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | - |
Aspergillus niger | cellobiose + cellotriose + ? | products are 18% cellotriose, 72% cellobiose and 10% glucose | ? | |
| 3.2.1.4 | cellobiose + H2O | - |
Aspergillus niger | ? | 9.8% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | cellobiose + H2O | - |
Aspergillus niger HO | ? | 9.8% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | filter paper + H2O | - |
Aspergillus niger | ? | 18% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | additional information | no substrate: cellulose | Aspergillus niger | ? | - |
? | |
| 3.2.1.4 | additional information | no substrate: cellulose | Aspergillus niger HO | ? | - |
? | |
| 3.2.1.4 | xylan + H2O | - |
Aspergillus niger | ? | 20% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | xylan + H2O | - |
Aspergillus niger HO | ? | 20% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | xyloglucan + H2O | - |
Aspergillus niger | ? | 71% of the activity with carboxymethyl cellulose | ? | |
| 3.2.1.4 | xyloglucan + H2O | - |
Aspergillus niger HO | ? | 71% of the activity with carboxymethyl cellulose | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | ? | x * 55000, SDS-PAGE | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | EglA | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 70 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 70 | - |
half-life 3 h | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 3.5 | - |
- |
Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 2 | - |
about 75% of maximum activity | Aspergillus niger |
| 3.2.1.4 | 8 | - |
about 10% of maximum activity | Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 3.5 | - |
3 h, no loss of activity | Aspergillus niger |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
