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Literature summary extracted from

  • Hansen, L.H.; Vester, B.
    A cfr-like gene from Clostridium difficile confers multiple antibiotic resistance by the same mechanism as the cfr gene (2015), Antimicrob. Agents Chemother., 59, 5841-5843.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.224 gene cfr, The Cfr-like protein in Peptoclostridium difficile is hosted by a transposon, and the same gene is found in other strains, sequence comparisons, overview. Recombinant expression in Escherichia coli strain AS19 which then shows elevated MIC values for five classes of antibiotics, and recombinant expression in rlmN-deficient Escherichia coli strain JW2505-1 Clostridioides difficile

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin Clostridioides difficile
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.224 Clostridioides difficile V5ZEZ1 formerly Clostridium difficile, gene cfr
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.224 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin
-
 Clostridioides difficile S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.224 Cfr
-
 Clostridioides difficile
2.1.1.224 Cfr-like protein
-
 Clostridioides difficile

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.224 Ferredoxin
-
 Clostridioides difficile
2.1.1.224 S-adenosyl-L-methionine
-
 Clostridioides difficile

General Information

EC Number General Information Comment Organism
2.1.1.224 physiological function the Cfr RNA methyltransferase causes multiple resistances to peptidyl transferase inhibitors by methylation of A2503 23S rRNA Clostridioides difficile