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Literature summary extracted from

  • Huang, J.; Chen, L.; Hu, N.; Jiang, W.; Wu, G.; Liu, Z.
    Characterization of a novel serine hydroxymethyltransferase isolated from marine bacterium Arthrobacter sp. and its application on L-serine production (2015), Ann. Microbiol., 65, 1689-1698.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 gene glyA, DNA and amino acid sequence determination and analysis, thermal asymmetric interlaced PCR, TAIL-PCR, sequence comparisons and phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 Co2+ strong inhibition at 1 mM Arthrobacter sp.
2.1.2.1 CTAB
-
Arthrobacter sp.
2.1.2.1 Cu2+ strong inhibition at 1 mM Arthrobacter sp.
2.1.2.1 Fe2+ strong inhibition at 1 mM Arthrobacter sp.
2.1.2.1 Hg2+ strong inhibition at 1 mM Arthrobacter sp.
2.1.2.1 Mn2+ strong inhibition at 1 mM Arthrobacter sp.
2.1.2.1 SDS
-
Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.1 Ca2+ activates slightly Arthrobacter sp.
2.1.2.1 EDTA activates slightly Arthrobacter sp.
2.1.2.1 Mg2+ activates slightly Arthrobacter sp.
2.1.2.1 additional information K+, Na+ and NH4+ show no appreciable impact on the SHMT activity Arthrobacter sp.
2.1.2.1 Pb2+ activates slightly Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Arthrobacter sp.
-
tetrahydrofolate + L-serine
-
r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Arthrobacter sp. MCCC 1A05493
-
tetrahydrofolate + L-serine
-
r
2.1.2.1 tetrahydrofolate + L-serine Arthrobacter sp.
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
2.1.2.1 tetrahydrofolate + L-serine Arthrobacter sp. MCCC 1A05493
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Arthrobacter sp.
-
gene glyA
-
2.1.2.1 Arthrobacter sp. MCCC 1A05493
-
gene glyA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Arthrobacter sp. tetrahydrofolate + L-serine
-
r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Arthrobacter sp. MCCC 1A05493 tetrahydrofolate + L-serine
-
r
2.1.2.1 tetrahydrofolate + L-serine
-
Arthrobacter sp. 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
2.1.2.1 tetrahydrofolate + L-serine
-
Arthrobacter sp. MCCC 1A05493 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
Arthrobacter sp.
2.1.2.1 SHMT
-
Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 45
-
-
Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 7.8
-
-
Arthrobacter sp.

General Information

EC Number General Information Comment Organism
2.1.2.1 evolution the enzyme belongs to the alpha-family of fold type I, and pyridoxal 5'-phosphate-dependent enzymes Arthrobacter sp.
2.1.2.1 physiological function SHMT plays an important role in the assimilation of C1 compounds, yielding the main L-serine intermediate Arthrobacter sp.