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Literature summary extracted from

  • Balaev, V.V.; Lashkov, A.A.; Gabdulkhakov, A.G.; Dontsova, M.V.; Seregina, T.A.; Mironov, A.S.; Betzel, C.; Mikhailov, A.M.
    Structural investigation of the thymidine phosphorylase from Salmonella typhimurium in the unliganded state and its complexes with thymidine and uridine (2016), Acta Crystallogr. Sect. F, 72, 224-233.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.4 expressed in Escherichia coli BL21(DE3) cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.4 sitting drop vapor diffusion method, using 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane, 20% (w/v) PEG 3350 pH 7.5 (unliganded enzyme), or 0.1 M Bicine, 1.6 M (NH4)2SO4 pH 9.0 (in complex with thymidine), or 0.1 M citric acid, 10% (w/v) PEG 6000 pH 5.0 (in complex with uridine) Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.4 47000
-
 x * 47000, calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 48000
-
 x * 48000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.4 thymidine + phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.4 Salmonella enterica subsp. enterica serovar Typhimurium Q7CP66
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.4 Ni Sepharose 6 column chromatography and Superdex 200 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.4 thymidine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.4 ? x * 48000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 ? x * 47000, calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
2.4.2.4 deoA
-
 Salmonella enterica subsp. enterica serovar Typhimurium