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Literature summary extracted from

  • Hou, H.; Li, R.; Wang, X.; Yuan, Z.; Liu, X.; Chen, Z.; Xu, X.
    Crystallographic analysis of a novel aldo-keto reductase from Thermotoga maritima in complex with NADP+ (2015), Acta Crystallogr. Sect. F, 71, 847-855.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.184 in complex with cofactor NADP+ and with NADP+ and substrate ethyl 2-oxo-4-phenylbutyrate, to 1.7 and 2.0 A resolution, respectively. Both crystals belong to space group P3121, with similar unit-cell parameters Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 35000
-
 x * 35000, SDS-PAGE, x * 35032, calculated Thermotoga maritima
1.1.1.184 35032
-
 x * 35000, SDS-PAGE, x * 35032, calculated Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Thermotoga maritima Q9X265
-
-
1.1.1.184 Thermotoga maritima DSM 3109 Q9X265
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 ethyl 2-oxo-4-phenylbutyrate + NADPH + H+
-
 Thermotoga maritima ethyl 2-hydroxy-4-phenylbutyrate + NADP+
-
 ?
1.1.1.184 ethyl 2-oxo-4-phenylbutyrate + NADPH + H+
-
 Thermotoga maritima DSM 3109 ethyl 2-hydroxy-4-phenylbutyrate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 ? x * 35000, SDS-PAGE, x * 35032, calculated Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 Tm_1743
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 60
-
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH
-
 Thermotoga maritima