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Literature summary extracted from

  • Niiyama, M.; Sugiyama, S.; Hirose, M.; Ishikawa, S.; Tomitori, H.; Higashi, K.; Yamashita, T.; Adachi, H.; Takano, K.; Murakami, S.; Murata, M.; Inoue, T.; Mori, Y.; Kashiwagi, K.; Matsumura, H.; Igarashi, K.
    Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli (2013), Acta Crystallogr. Sect. F, 69, 884-887.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.57 gene speG, recombinant enzyme expression in Escherichia coli strain BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.57 purified recombinant enzyme, sitting-drop vapour-diffusion method, mixing of 0.001 ml of 40 mg/ml protein in 10 mM HEPES-KOH pH 7.5, 50 mM CoA, 50 mM spermidine, 300 mM KCl, 0.1 mM EDTA, 6 mM 2-mercaptoethanol, 10% glycerol, 10 mM PMSF, 0.02% Brij-35, with 0.001 ml of reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 9% w/v PEG 8000, 0.1 M calcium acetate, and equilibration against 0.5 ml reservoir solution, 20°C, a few days, X-ray diffraction structure determination and analysis at 2.5 A resolution Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.57 22000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.57 3 acetyl-CoA + spermidine Escherichia coli
-
 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Escherichia coli
-
 gene speG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57 recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and concanavalin A affinity chromatography, followed by gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 3 acetyl-CoA + spermidine
-
 Escherichia coli 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.57 SAT
-
 Escherichia coli
2.3.1.57 spermidine acetyltransferase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.57 acetyl-CoA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.3.1.57 physiological function a key enzyme that transfers the acetyl group from acetyl-CoA to either the N-1 or N-8 position of spermidine, thereby reducing the intracellular polyamine level Escherichia coli