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Literature summary extracted from

  • Janardan, N.; Harijan, R.K.; Kiema, T.R.; Wierenga, R.K.; Murthy, M.R.
    Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis (2015), Acta Crystallogr. Sect. D, 71, 2479-2493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9 expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.9 microbatch method, using either 20% (w/v) PEG 3350, 0.15 M calcium chloride dehydrate, or 45% (w/v) PEG 200, 0.1 M MES monohydrate pH 6.0, 0.07 M calcium chloride dehydrate or 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, 30% (w/v) PEG 8000, 5% (v/v) n-octyl-beta-D-glucoside Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 2 acetyl-CoA Mycolicibacterium smegmatis
-
 CoA + acetoacetyl-CoA
-
 ir
2.3.1.9 additional information Mycolicibacterium smegmatis no activity is observed with acetyl-CoA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 Ni2+-NTA bead chromatography and Superose 6 gel filtration Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 2 acetyl-CoA
-
 Mycolicibacterium smegmatis CoA + acetoacetyl-CoA
-
 ir
2.3.1.9 additional information no activity is observed with acetyl-CoA Mycolicibacterium smegmatis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.9 homotetramer x-ray crystallography Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.9 3-ketoacyl-CoA (T1)-like thiolase
-
 Mycolicibacterium smegmatis
2.3.1.9 MSM-13 thiolase
-
 Mycolicibacterium smegmatis