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Literature summary extracted from

  • Chitnumsub, P.; Jaruwat, A.; Riangrungroj, P.; Ittarat, W.; Noytanom, K.; Oonanant, W.; Vanichthanankul, J.; Chuankhayan, P.; Maenpuen, S.; Chen, C.J.; Chaiyen, P.; Yuthavong, Y.; Leartsakulpanich, U.
    Structures of Plasmodium vivax serine hydroxymethyltransferase: implications for ligand-binding specificity and functional control (2014), Acta Crystallogr. Sect. D, 70, 3177-3186.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 purified enzyme in a binary complex with L-serine and in a ternary complex with D-serine and (6R)-5-formyltetrahydrofolate, microbatch method, mixing of 0.001 ml of 0.38 mM protein in 0.86 mM PLP, 62 mM 2-mercaptoethanol, 87 mM Gly, L-Ser, or D-Ser, and 43 mM tetrahydrofolate, with 0.001 ml of well solution containing 20-21% w/v PEG 4000, 70-90 mM NaCl, 100 mM Tris-HCl, pH 8.5, 15% v/v trifluoroethanol, 20°C, 1-4 days, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution, molecular replacement Plasmodium vivax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 additional information Plasmodium vivax the enzyme catalyzes the conversion of L- and D-serine to glycine in a THFdependent reaction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Plasmodium vivax A5K8L9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Plasmodium vivax tetrahydrofolate + L-serine
-
 r
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Plasmodium vivax tetrahydrofolate + D-serine
-
 r
2.1.2.1 additional information the enzyme catalyzes the conversion of L- and D-serine to glycine in a THFdependent reaction Plasmodium vivax ?
-
 ?
2.1.2.1 additional information L- and D-serine substrate binding structure analysis, overview Plasmodium vivax ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Plasmodium vivax
2.1.2.1 SHMT
-
 Plasmodium vivax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 25
-
 assay at Plasmodium vivax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 8
-
 assay at Plasmodium vivax

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 5,10-methylenetetrahydrofolate
-
 Plasmodium vivax
2.1.2.1 pyridoxal 5'-phosphate
-
 Plasmodium vivax
2.1.2.1 tetrahydrofolate binding structure analysis Plasmodium vivax

General Information

EC Number General Information Comment Organism
2.1.2.1 metabolism the enzyme is involved in folate recycling and dTMP synthesis Plasmodium vivax