EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.7.1.8 | S112A and H265Q mutants crystal structure analysis, PDB IDs 2PUH and 3V1N, modeling | Paraburkholderia xenovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.7.1.8 | 2,6-dioxo-6-phenylhexa-3-enoate + H2O | Paraburkholderia xenovorans | - |
benzoate + 2-oxopent-4-enoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.7.1.8 | Paraburkholderia xenovorans | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.7.1.8 | 2,6-dioxo-6-phenylhexa-3-enoate + H2O = benzoate + 2-oxopent-4-enoate | catalytic mechanism involving enol-to-keto tautomerization that consists of two elementary reactions, the calculated Boltzmann weighted average barriers favor a substrate-assisted acylation mechanism, and the most feasible acylation pathway involves a catalytic triad, Ser-His-Asp. The product (2-hydroxypenta-2,4-dienoic acid) of the acylation process is replaced by three water molecules, and one of which is involved in the deacylation process, quantum mechanics/molecular mechanics study, overview | Paraburkholderia xenovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.7.1.8 | 2,6-dioxo-6-phenylhexa-3-enoate + H2O | - |
Paraburkholderia xenovorans | benzoate + 2-oxopent-4-enoate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.7.1.8 | BphD | - |
Paraburkholderia xenovorans |
3.7.1.8 | meta-cleavage product hydrolase | - |
Paraburkholderia xenovorans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.7.1.8 | evolution | BphD belongs to meta-cleavage product (MCP) hydrolases which possesses an alpha/beta-hydrolase fold and utilizes a Ser-His-Asp triad during catalysis | Paraburkholderia xenovorans |
3.7.1.8 | metabolism | meta-cleavage product hydrolase BphD is the fourth enzyme of the biphenyl catabolic pathway | Paraburkholderia xenovorans |
3.7.1.8 | additional information | energy profiles of three individual pathways along substrate-assisted acylation (Ser112-His265-Asp237 involved) and deacylation pathways | Paraburkholderia xenovorans |