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Literature summary extracted from

  • Felux A.-K, F.A.; Spiteller D, S.D.; Klebensberger J, K.J.; Schleheck D, S.D.
    Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1 (2015), Proc. Natl. Acad. Sci. USA, 112, E4298-E4305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.390 expressed in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.390 0.5
-
 sulfoquinovose with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.1.1.390 2.4
-
 sulfoquinovose with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.2.1.97 0.14
-
 (2S)-3-sulfolactaldehyde with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.2.1.97 0.14
-
 (2S)-3-sulfolactaldehyde with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.390 additional information Pseudomonas putida the enzyme shows no activity with D-glucose-6-phosphate ?
-
 ?
1.1.1.390 additional information Pseudomonas putida SQ1 the enzyme shows no activity with D-glucose-6-phosphate ?
-
 ?
1.1.1.390 sulfoquinovose + NAD+ Pseudomonas putida sulfoquinovose is 6-deoxy-6-sulfoglucose 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.390 sulfoquinovose + NAD+ Pseudomonas putida SQ1 sulfoquinovose is 6-deoxy-6-sulfoglucose 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NAD+ + H2O Pseudomonas putida
-
 (2S)-3-sulfolactate + NADH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NAD+ + H2O Pseudomonas putida SQ1
-
 (2S)-3-sulfolactate + NADH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NADP+ + H2O Pseudomonas putida
-
 (2S)-3-sulfolactate + NADPH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NADP+ + H2O Pseudomonas putida SQ1
-
 (2S)-3-sulfolactate + NADPH + H+
-
 ?
3.1.1.99 6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O Pseudomonas putida
-
 6-deoxy-6-sulfo-D-gluconate
-
 ?
4.2.1.162 6-deoxy-6-sulfo-D-gluconate Pseudomonas putida
-
 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.390 Pseudomonas putida
-
-
-
1.1.1.390 Pseudomonas putida SQ1
-
-
-
1.2.1.97 Pseudomonas putida
-
-
-
1.2.1.97 Pseudomonas putida SQ1
-
-
-
3.1.1.99 Pseudomonas putida
-
-
-
3.1.1.99 Pseudomonas putida SQ1
-
-
-
4.2.1.162 Pseudomonas putida
-
-
-
4.2.1.162 Pseudomonas putida SQ1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.390 Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.390 additional information the enzyme shows no activity with D-glucose-6-phosphate Pseudomonas putida ?
-
 ?
1.1.1.390 additional information the enzyme shows no activity with D-glucose-6-phosphate Pseudomonas putida SQ1 ?
-
 ?
1.1.1.390 sulfoquinovose + NAD+ sulfoquinovose is 6-deoxy-6-sulfoglucose Pseudomonas putida 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.390 sulfoquinovose + NAD+ sulfoquinovose is 6-deoxy-6-sulfoglucose Pseudomonas putida SQ1 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.390 sulfoquinovose + NADP+ when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ Pseudomonas putida 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.390 sulfoquinovose + NADP+ when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ Pseudomonas putida SQ1 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NAD+ + H2O
-
 Pseudomonas putida (2S)-3-sulfolactate + NADH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NAD+ + H2O
-
 Pseudomonas putida SQ1 (2S)-3-sulfolactate + NADH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NADP+ + H2O
-
 Pseudomonas putida (2S)-3-sulfolactate + NADPH + H+
-
 ?
1.2.1.97 (2S)-3-sulfolactaldehyde + NADP+ + H2O
-
 Pseudomonas putida SQ1 (2S)-3-sulfolactate + NADPH + H+
-
 ?
3.1.1.99 6-deoxy-6-sulfo-D-glucono-1,5-lactone + H2O
-
 Pseudomonas putida 6-deoxy-6-sulfo-D-gluconate
-
 ?
4.2.1.162 6-deoxy-6-sulfo-D-gluconate
-
 Pseudomonas putida 2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.390 SQ dehydrogenase
-
 Pseudomonas putida
1.2.1.97 SLA dehydrogenase
-
 Pseudomonas putida
3.1.1.99 6-deoxy-6-sulfogluconolactone lactonase
-
 Pseudomonas putida
3.1.1.99 SGL lactonase
-
 Pseudomonas putida
4.2.1.162 SG dehydratase
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.390 1.4
-
 sulfoquinovose with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.1.1.390 33.8
-
 sulfoquinovose with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.2.1.97 11.4
-
 (2S)-3-sulfolactaldehyde with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.2.1.97 11.8
-
 (2S)-3-sulfolactaldehyde with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.390 NAD+ dependent on Pseudomonas putida
1.1.1.390 NADP+ when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ Pseudomonas putida
1.2.1.97 NAD+
-
 Pseudomonas putida
1.2.1.97 NADP+
-
 Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.390 0.6
-
 sulfoquinovose with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.1.1.390 67.6
-
 sulfoquinovose with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.2.1.97 81.4
-
 (2S)-3-sulfolactaldehyde with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
1.2.1.97 84.2
-
 (2S)-3-sulfolactaldehyde with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida