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Literature summary extracted from

  • Jin, J.; Wu, R.; Zhu, J.; Yang, S.; Lei, Z.; Wang, N.; Singh, V.K.; Zheng, J.; Jia, Z.
    Insights into the cellular function of YhdE, a nucleotide pyrophosphatase from Escherichia coli (2015), PLoS ONE, 10, e0117823.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.9 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.9 mutant E33A, hanging drop vapor diffusion method, using 0.1 Mmagnesium sulfate, 0.1 M MES buffer (pH 6.5), and 10-15% (w/v) PEG 8000 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.9 E33A inactive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.09
-
 dTTP at pH 7.0 and 25°C Escherichia coli
3.6.1.9 0.5
-
 UTP at pH 7.0 and 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 45000
-
 2 * 45000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Escherichia coli P25536
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 Ni-charged column chromatography and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 dTTP + H2O the enzyme possesses dTTP-specific activity at a substrate concentration of 0.1 mM Escherichia coli dTMP + diphosphate
-
 ?
3.6.1.9 dUTP + H2O weakest substrate Escherichia coli dUMP + diphosphate
-
 ?
3.6.1.9 additional information no activity with ATP, CTP, GTP, TDP, TMP, and dCTP Escherichia coli ?
-
 ?
3.6.1.9 TTP + H2O the enzyme exhibits significant activity for TTP only when substrate concentration reaches 0.5 mM Escherichia coli TMP + diphosphate
-
 ?
3.6.1.9 UTP + H2O the enzyme exhibits significant activity for UTP only when substrate concentration reaches 0.5 mM Escherichia coli UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.9 homodimer 2 * 45000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.6.1.9 Maf-like protein YhdE
-
 Escherichia coli
3.6.1.9 nucleotide pyrophosphatase
-
 Escherichia coli
3.6.1.9 PPase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.9 43
-
 dTTP at pH 7.0 and 25°C Escherichia coli
3.6.1.9 95
-
 UTP at pH 7.0 and 25°C Escherichia coli

General Information

EC Number General Information Comment Organism
3.6.1.9 malfunction enzyme overexpression results in filamentous cells. The enzyme plays a role in in cell growth inhibition under stress conditions, cell-division arrest and cell-shape maintenance Escherichia coli
3.6.1.9 physiological function emzyme-knockout cells transform the normal rod shape of wild type cells to a more spherical form, and the cell wall appears to become more flexible Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.9 191
-
 UTP at pH 7.0 and 25°C Escherichia coli
3.6.1.9 503
-
 dTTP at pH 7.0 and 25°C Escherichia coli