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Literature summary extracted from

  • Chen, M.; Markham, J.; Cahoon, E.
    Sphingolipid DELTA8 unsaturation is important for glucosylceramide biosynthesis and low-temperature performance in Arabidopsis (2012), Plant J., 69, 769-781.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.29 expressed in Saccharomyces cerevisiae BY4741 cells Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.29 endoplasmic reticulum
-
Arabidopsis thaliana 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Arabidopsis thaliana
-
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O the ratio of trans:cis products is approximately 4:1 for isoform SLD1 and 7:1 for isoform SLD2 ?
1.14.19.29 sphinganine ceramide + ferrocytochrome b5 + O2 + H+ Arabidopsis thaliana
-
8-sphingenine ceramide + ferricytochrome b5 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.29 Arabidopsis thaliana
-
ecotype Col-0
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.29 flower isoform SLD1 and SLD2 are most highly expressed in the flower Arabidopsis thaliana
-
1.14.19.29 leaf isoform SLD1 Arabidopsis thaliana
-
1.14.19.29 root isoform SLD1 Arabidopsis thaliana
-
1.14.19.29 silique isoform SLD1 and SLD2 Arabidopsis thaliana
-
1.14.19.29 stem isoform SLD1 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Arabidopsis thaliana (4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O the ratio of trans:cis products is approximately 4:1 for isoform SLD1 and 7:1 for isoform SLD2 ?
1.14.19.29 sphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Arabidopsis thaliana 8-sphingenine ceramide + ferricytochrome b5 + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.29 DELTA8 Des
-
Arabidopsis thaliana
1.14.19.29 LCB DELTA8 desaturase
-
Arabidopsis thaliana
1.14.19.29 SLD1 isoform Arabidopsis thaliana
1.14.19.29 SLD2 isoform Arabidopsis thaliana
1.14.19.29 sphingoid long-chain base DELTA8 desaturase
-
Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.29 cytochrome b5
-
Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.19.29 malfunction enzyme deletion mutants reveal a 50% reduction in glucosylceramide levels and a corresponding increase in glycosylinositolphosphoceramides Arabidopsis thaliana