Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Morikawa, T.; Mizutani, M.; Aoki, N.; Watanabe, B.; Saga, H.; Saito, S.; Oikawa, A.; Suzuki, H.; Sakurai, N.; Shibata, D.; Wadano, A.; Sakata, K.; Ohta, D.
    Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato (2006), Plant Cell, 18, 1008-1022.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.41 expressed in a baculovirus/Sf9 insect cell system Arabidopsis thaliana
1.14.19.41 expressed in a baculovirus/Sf9 insect cell system Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.41 0.001
-
 beta-sitosterol at pH 7.3 and 30°C Arabidopsis thaliana
1.14.19.41 0.0037
-
 beta-sitosterol at pH 7.3 and 30°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.41 microsome
-
 Arabidopsis thaliana
-
-
1.14.19.41 microsome
-
 Solanum lycopersicum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.41 55000
-
 x * 55000, SDS-PAGE Arabidopsis thaliana
1.14.19.41 55000
-
 x * 55000, SDS-PAGE Solanum lycopersicum
1.14.19.41 55723
-
 x * 55723, calculated from amino acid sequence Arabidopsis thaliana
1.14.19.41 57511
-
 x * 57511, calculated from amino acid sequence Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.41 Arabidopsis thaliana
-
 ecotype Columbia
-
1.14.19.41 Solanum lycopersicum Q1T7C2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.41 24-epi-campesterol + NADPH + H+ + O2
-
 Arabidopsis thaliana brassicasterol + NADP+ + H2O
-
 ?
1.14.19.41 beta-sitosterol + NADPH + H+ + O2
-
 Arabidopsis thaliana stigmasterol + NADP+ + H2O
-
 ?
1.14.19.41 beta-sitosterol + NADPH + H+ + O2
-
 Solanum lycopersicum stigmasterol + NADP+ + H2O
-
 ?
1.14.19.41 additional information no activity with campesterol Arabidopsis thaliana ?
-
 ?
1.14.19.41 additional information no activity with campesterol Solanum lycopersicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.41 ? x * 55000, SDS-PAGE Arabidopsis thaliana
1.14.19.41 ? x * 55000, SDS-PAGE Solanum lycopersicum
1.14.19.41 ? x * 55723, calculated from amino acid sequence Arabidopsis thaliana
1.14.19.41 ? x * 57511, calculated from amino acid sequence Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
1.14.19.41 CYP710A
-
 Arabidopsis thaliana
1.14.19.41 CYP710A11
-
 Solanum lycopersicum
1.14.19.41 sterol C-22 desaturase
-
 Arabidopsis thaliana
1.14.19.41 sterol C-22 desaturase
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.41 0.009
-
 beta-sitosterol at pH 7.3 and 30°C Arabidopsis thaliana
1.14.19.41 0.17
-
 beta-sitosterol at pH 7.3 and 30°C Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.41 NADPH
-
 Arabidopsis thaliana
1.14.19.41 NADPH
-
 Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.19.41 8.8
-
 beta-sitosterol at pH 7.3 and 30°C Arabidopsis thaliana
1.14.19.41 46.7
-
 beta-sitosterol at pH 7.3 and 30°C Solanum lycopersicum