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Literature summary extracted from

  • Kakugawa, S.; Langton, P.F.; Zebisch, M.; Howell, S.A.; Chang, T.H.; Liu, Y.; Feizi, T.; Bineva, G.; OReilly, N.; Snijders, A.P.; Jones, E.Y.; Vincent, J.P.
    Notum deacylates Wnt proteins to suppress signalling activity (2015), Nature, 519, 187-192.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.98 nine crystal forms at resolutions between 1.4 and 2.8 A. The catalytic triad comprises Ser232, Asp340 and His389 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.98 Phenylmethanesulfonylfluoride strong inhibition of 4-nitrophenylacetate hydrolysis Homo sapiens
3.1.1.98 Triton X-100 strong inhibition of 4-nitrophenylacetate hydrolysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.98 Drosophila melanogaster Q9VUX3
-
-
3.1.1.98 Homo sapiens Q6P988
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.98 4-nitrophenyl acetate + H2O
-
 Homo sapiens 4-nitrophenol + acetate
-
 ?
3.1.1.98 additional information no measurable activity on representative sulfatase, phosphatase, phospholipase C or amidase/protease substrates Homo sapiens ?
-
 ?
3.1.1.98 [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H2O
-
 Drosophila melanogaster [Wnt]-L-serine + (9Z)-hexadec-9-enoate Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins ?
3.1.1.98 [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H2O
-
 Homo sapiens [Wnt]-L-serine + (9Z)-hexadec-9-enoate Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins ?

General Information

EC Number General Information Comment Organism
3.1.1.98 physiological function Notum requires glypicans to suppress Wnt signalling, but does not cleave their glycophosphatidylinositol anchor. Glycosaminoglycan binding sites on Notum probably help to co-localize with Wnt proteins. A large hydrophobic pocket at the active site accommodates palmitoleate Drosophila melanogaster
3.1.1.98 physiological function Notum requires glypicans to suppress Wnt signalling, but does not cleave their glycophosphatidylinositol anchor. Glycosaminoglycan binding sites on Notum probably help to co-localize with Wnt proteins. A large hydrophobic pocket at the active site accommodates palmitoleate Homo sapiens